Multiple Immunostaining Methods to Detect Traumatic Axonal Injury in the Rat Fluid-Percussion Brain Injury Model
スポンサーリンク
概要
- 論文の詳細を見る
Immunohistochemistry using beta-amyloid precursor protein (APP) N-terminus antibodies is routinely used to detect traumatic axonal injury (TAI). The temporal and regional distributions of APP C- and N-terminus immunoreactivity were investigated in rats with experimental brain injury and compared to distribution of neurofilament (NF) immunoreactivity. Male Sprague-Dawley rats underwent right later-al fluid-percussion (FP) brain injury or sham injury. Six FP injury rats and two control rats were trans-cardially fixed with 10% formalin at 1, 6, 24, and 48 hours, and 1 and 2 weeks after injury and serial6μm-thick paraffin sections were prepared. At 6 hours after injury, APP C-terminus immunostaining labeled small neurons and axonal deposits in the injured parasagittal cortex, striatum, thalami, and dorsal medulla, whereas APP N-terminus and NF immunostaining showed few axonal deposits in the parasagittal cortex. At 24-48 hours post-injury, marked axonal damage, including axonal swelling and bulbs, was observed in the injured cerebral hemisphere, cerebellar white matter, and medulla. NF immunostaining was most sensitive for axonal damage in the injured deep cortical layers, cerebellum, and medulla. At 1-2 weeks after injury, APP N-terminus immunostaining mainly showed dot-like axonal profiles in the injured thalamus. APP C-terminus immunoreactivity may serve as an early marker of TAI, and the C-terminal fragments of APP may be involved in the evolution of TAI because C-terminal fragments of APP are neurotoxic and pro-apoptotic. Multiple immunostaining methods may be required to fully recognize the extent of TAI.
- 日本脳神経外科学会の論文
- 2003-04-15
著者
-
TERAMOTO Akira
Departments of Neurosurgery, Nippon Medical School
-
Asakura Takayuki
Department Of Neurosurgery Nippon Medical School
-
Teramoto Akira
Department Of Neurosurgery Nippon Medical School
-
KOBAYASHI Shiro
Department of Neurosurgery, Chiba Hokusoh Hospital, Nippon Medical School
-
HOSHINO Shigeru
Department of Neurosurgery, Nippon Medical School
-
FURUKAWA Tetsuya
Department of Neurosurgery, Nippon Medical School, Chiba Hokusoh Hospital
-
Hoshino Shigeru
Department Of Neurosurgery Nippon Medical School
-
Hoshino Shigeru
Department Internal Medicine Saitama Red Cross Hospital
-
Kobayashi Shiro
Department Of Neurosurgery Chiba Hokuso Hospital Nippon Medical School
-
Kobayashi Shiro
Department Of Material Chemistry Graduate School Of Engineering Kyoto University
-
Teramoto Akira
Department Of Functional Polymer Science Shinshu University
-
Kobayashi Shiro
Department Of Neurosurgery Nippon Medical School Chiba Hokusoh Hospital
-
Furukawa Tetsuya
Department Of Neurosurgery Mejiro Third Hospital
関連論文
- Ruptured Aneurysm of the Orbitofrontal Artery Associated With Dural Arteriovenous Malformation in the Anterior Cranial Fossa : Case Report
- Central Diabetes Insipidus after Transsphenoidal Surgery in Dogs with Cushings Disease
- Efficacy of Transsphenoidal Surgery on Endocrinological Status and Serum Chemistry Parameters in Dogs with Cushings Disease
- Administration of Free Radical Scavenger Edaravone Associated With Higher Frequency of Hemorrhagic Transformation in Patients With Cardiogenic Embolism
- Efficacy of Edaravone, a Free Radical Scavenger, for the Treatment of Acute Lacunar Infarction
- Benzodiazepine receptor imaging with iomazenil SPECT in aphasic patients with cerebral infarction
- Malignant Fibrous Histiocytoma in the Parietal Bone : Case Report
- Production of Pituitary Hormone by Human Pituitary Adenoma is under Autocrine and Paracrine Regulation of Hypothalamic Hormones Secreted from Adenoma Cells(Prof. K Watanabe Memorial Article)
- Histopathological Review of Silent Pituitary Somatotroph Adenoma
- P4-22 Functional Differentiation and Transcription Factors in Human Non-Functioning Pituitary Adenomas
- P4-21 Immunohistochemical Study of Neural Stem Cell Marker Nestin in Human Pitutary Glands and Pituitary
- Expression of Neuro D1 in Human Nontumorous Pituitaries and Pituitary Adenomas
- Mechanism of Hormone Production in Pituitary Cells and Pituitary Neoplasms; Synergistic Actions of Transcription Factors (Symposium IRegulation of Endocrine Function)
- Silent Somatotroph Adenoma, Detected by Catalyzed Signal Ampligication and Non-radioisotopic In sity Hybridization
- Functional Differentiation and its Regulation in Pituitary Cells (Recent Progress In Gene Histochemistry)
- P-56 Immunohistochemical Detection of Rab 3 Protein in the Pituitary Glands of the Rat Species and the GH-RH Transgenic Rat
- Concomitant Cranial and Lumbar Subdural Hematomas : Case Report
- Rupture of a Vertebral Artery Dissecting Aneurysm Developing Immediately After Trapping of a Dissecting Aneurysm on the Contralateral Vertebral Artery : Case Report
- Intracerebral Hemorrhage Caused by a Neoplastic Aneurysm From Pleomorphic Lung Carcinoma
- Elevation of Growth Hormone-Releasing Hormone Receptor Messenger Ribonucleic Acid Expression in Growth Hormone-Secreting Pituitary Adenoma With Gsα Protein Mutation
- Cervical Ligamentum Flavum Ossification
- Middle Cerebral Artery Thrombolysis Through the Contralateral Internal Carotid Artery : Case Report
- Effect of Prostaglandin E_1 on Cerebral Blood Flow in Patients with Chronic Cerebral Infarction
- Persistent Primitive Hypoglossal Artery Complicated by Atrial Septal Defect and Congenital Intrahepatic Shunts
- Cavernous Angioma with Olivary Hypertrophy and Contralateral Cerebellar Diaschisis
- Magnetic Resonance Imaging Assessment of Pituitary Posterior Lobe Displacement in Dogs with Pituitary-Dependent Hyperadrenocorticism
- Posttraumatic Carotid-Cavernous Fistulae Treated by Internal Carotid Artery Trapping and High-Flow Bypass Using a Radial Artery Graft : —Two Case Reports—
- Coexistence of Corticotroph Adenoma and Thyrotroph Hyperplasia in a Dog(Surgery)
- Osteosarcoma Arising From the Skull
- Mechanisms for Production and Secretion of Hormones in Physiologic and Pathologic Conditions
- Biodegradability of Poly (L-lactic Acid) Functionally Graded Materials with Hardness Gradient
- Surgery: Central diabetes insipidus after transsphenoidal surgery in dogs with cushing's disease
- Sexually Dimorphic Modulation of GABA_A Receptor Currents by Melatonin in Rat Gonadotropin-Releasing Hormone Neurons
- Multiple Immunostaining Methods to Detect Traumatic Axonal Injury in the Rat Fluid-Percussion Brain Injury Model
- A Ten-Year Old Male with Bromocriptine Resistant PRL and GH-Producing Huge Pituitary Adenoma
- Anti-Alpha-Enolase Antibodies in Pituitary Disease
- Hypophysectomy for a Dog with Coexisting Cushings Disease and Diabetes Mellitus
- Mechanism of D_2 Agonist-induced Inhibition of GH Secretion from Human GH-secreting Adenoma Cells
- Mechanism of Adrenomedullin-induced Prolactin Release from Human Prolactin-releasing Adenoma Cells
- Mechanisms of TRH-induced GH Release (Paradoxical Response) in Human Somatotroph Adenoma Cells
- A Study of Anterior Pituitary Hormones Secretion in Patients with Glioma Receiving Interferon-Beta Treatment
- Extracranially extended meningothelial meningiomas with a high MIB-1 index : A report of two cases
- Severe Papilledema Identified 3 Weeks After Head Injury
- Hemangioblastomas with Blood Supply from the Dural Arteries : Two Case Reports
- Intracranial Osteosarcoma After Radiosurgery : Case Report
- Central neurocytoma expressing characteristics of ependymal differentiation : electron microscopic findings of two cases
- Delayed Recurrent Arachnoid Cyst of the Occipital Convexity in an Elderly Woman
- Paramedian Suboccipital Mini-Craniectomy for Evacuation of Spontaneous Cerebellar Hemorrhage
- Pituitary Stone : Case Report
- Bilateral Traumatic Abducens Nerve Palsy Associated With Skull Base Fracture : Case Report
- Increased Regional Interleukin-4 During the Acute Stage of Severe Intracranial Disorders
- Application of Genetic Engineering Technologies for the Study of Pituitary Development and Neoplasms
- Ab Initio Calculation on the Reaction Mechanism of "Radical-Controlled" Oxidative Polymerization of Phenols
- A family with spinal anaplastic ependymoma : evidence of loss of chromosome 22q in tumor
- Head-to-head juxtaposition of Fas-associated phosphatase-1 (FAP-1) and c-Jun NH2-terminal kinase 3 (JNK3) genes : genomic structure and seven polymorphisms of the FAP-1 gene
- Enzymatic Oxidative Polymerization of p-t-Butylphenol and Characterization of the Product Polymer
- High-Performance Immobilized Lipase Catalyst for Polyester Synthesis
- Lipase-Catalyzed Ring-Opening Polymerization of Substituted Lactones
- Structural Control in Enzymatic Oxidative Polymerization of Phenols with Varying the Solvent and Substituent Nature
- Enzymatic Synthesis of Polyesters in Ionic Liquids
- A New Crosslinking Method of Vinyl Polymers Having a Phenol Moiety via Oxidative Coupling
- Laccase-Catalyzed Curing of Vinyl Polymers Bearing a Phenol Moiety in the Side Chain
- Preparation of Artifical Urushi via an Environmentally Benign Process
- Lipase-Catalyzed Hydrolytic Degradation of Polyurethane in Organic Solvent
- Molecular Weight Control of Polyphenols by Enzymatic Copolymerization of Phenols
- Artificial Urushi: Design, Synthesis, and Enzymatic Curing of New Urushiol Analogues
- Synthesis and Curing of Crosslinkable Polyphenols from Urushiol Analogues
- Is technetium-99m-MIBI taken up by the normal pituitary gland? A comparison of normal pituitary glands and pituitary adenomas
- Enzymatic Synthesis and Curing of Poly (cardanol)
- リパーゼ
- Group Transfer Polymerization of Methacryl-Type Poly(2-oxazoline) Macromonomers
- Enzymatic Synthesis of Aromatic Polyesters by Lipase - Catalyzed Polymerization of Dicarboxylic Acid Divinyl Esters and Glycols
- Reactivity in Radical Polymerization of Poly(2 - oxazoline) Macromonomers
- Enzymatic Polymerization of Dicarboxylic Acid and Glycol to Polyester in Solvent-Free System
- Peroxidase-Catalyzed Dispersion Polymerization of Phenol Derivatives
- Extremely Efficient Catalysis of Immobilized Lipase in Ring-opening Polymerization of Lactones
- Mechanistic Studies on the Lipase-Catalyzed Ring-Opening Polymerization of Lactones
- Novel Bienzymatic Catalysis System for Oxidative Polymerization of Phenols
- Dehydration Polymerization in Aqueous Medium Catalyzed by Lipase
- Lipase - Catalyzed Ring - Opening Polymerization and Copolymerization of β - Propiolactone
- Enzymatic Synthesis and Thermal Properties of a New Class of Polyphenol
- Peroxidase-Catalyzed Oxidative Polymerization of Cresols to a New Family of Polyphenols
- One-Shot Synthesis of Polyester Macromonomer by Enzymatic Ring-Opening Polymerization of Lactone in the Presence of Vinyl Ester
- Preparation of Polyphenol Particles by Dispersion Polymerization Using Enzyme as Catalyst
- The c-Jun NH_2-terminal kinase3 (JNK3) gene : genomic structure, chromosomal assignment, and loss of expression in brain tumors
- Chronic Subdural Hematoma Following Dural Metastasis of Gastric Cancer: Measurement of Pre- and Postoperative Cerebral Blood Flow With N-isopropyl-p-[^^ I]iodoamphetamine : Case Report
- Suprasellar Ectopic Pituitary Adenoma : Case Report
- Immobilized Lipase Showing High Catalytic Activity toward Enzymatic Ring-Opening Polymerization of Macrolides
- Persistent Primitive Trigeminal Artery Aneurysm Associated With Cerebellar Hemangioblastoma : Case Report
- Regioselective Polymerization of Sorbitol and Divinyl Sebacate Using Lipase Catalyst
- Synthesis of Aliphatic Polyester by Insertion of Glycols into Polyanhydrides Using Lipase Catalyst
- Enzymatic Oxidation Polymerization of ο-Phenylenediamine
- Cardiopulmonary Hemodynamic Changes During Acute Subdural Hematoma Evacuation
- A family with hydrocephalus as a complication of cerebellar hemangioblastoma: identification of Pro157Leu mutation in the VHL gene
- Selective Posterior Decompression of the Cervical Spine
- Indocyanine Green Videoangiography of Optic Cavernous Angioma : Case Report
- Posttraumatic Carotid-Cavernous Fistulae Treated by Internal Carotid Artery Trapping and High-Flow Bypass Using a Radial Artery Graft : Two Case Reports
- Resection of Arteriovenous Malformation in a Patient With Hemophilia Type A
- Assessment of cerebral hemodynamics in childhood moyamoya disease using a quantitative and a semiquantitative IMP-SPECT study
- Detailed Study of Graft Sinking and Worsening of the Fused Segment Angle in Patients With Cervical Disease Treated With the Williams-Isu Method