血清中テオフィリン濃度測定におけるモノクロナール抗体を用いた螢光偏光免疫測定法の臨床的評価

概要

論文の詳細を見る
Theophylline concentration in serum was measured by fluorescence polarization immunoassay with monoclonal antibody (MC-FPIA). Within-run precision of the MC-FPIA method determined by 10 replicate analyses of 3 control sera gave coefficients of variation o f1.66, 1.81 and 1.48% for the low, medium and high control sera, respectively, while between-run coefficients of variation were 3.09, 3.07 and 3.49% for the low, medium and high control sera. The recovery of clinically relevant concentrations of theophylline-spiking drug-free plasma was 98.6 to 101.0%. Results of MC-FPIA on serum samples from asthmatic patients receiving theophylline were compared quantitatively with the results of high performance liquid chromatography. The correlation coefficient was 0.990. The cross-reactivity of 3 main human theophylline metabolites and 4 xanthine derivatives was evaluated and, as a results, no significant effect on the study result was noted. We conclude that MC-FPIA is a reliable and reproducible method for clinical theophylline level monitoring.
日本医療薬学会の論文
1985-08-20