高速液体クロマトグラフィーによる市販軟膏および混合製剤からの吉草酸ベタメタゾンの定量

概要

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High performance liquid chromatography (HPLC) was used in determination of betamethasone-17-valerate (BM-V) in commercial ointment. The conditions of HPLC were as follows-model: Shimadzu LC-4A ; detector : SPD-2SA; wave length : 239nm; column : Zorbax ODS (4.6 x 250mm); short guard-column : Zorbax ODS(4.0 x 50mm); mobile phase : MeOH: H_2O = 75 : 25(v/v); flow rate: 1 ml/min; pressure: 100kg/cm^2 (at 40℃). BM-V in commercial ointment and other ointment or cream admixture was also measured by the present HPLC method. BM-V ointment or admixture was dissolved or suspended into cyclohexane, and then MeOH : H_2O (9 : 1 or 8 : 2) was used to extract BM-V from cyclohexane. Naphthalene was used as an internal standard material. 10μl of extract solution was directly injected into HPLC column. The amount of BM-V was determined by comparing the peak height of BM-V to that of naphthalene on the chromatogram. The results showed the possibility that compatibility of BM-V ointment admixed with other ointment or cream can be studied with the present method.
日本医療薬学会の論文
1984-10-20