TDXジゴキシン測定用試薬による血清中デスラノシド濃度の測定

概要

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The use of a reagent for digoxin assay of fluorescence polarization immunoassay with TDX analyzer in the determination of serum deslanoside concentration was studied. Deslanoside calibrators with the concentration of 0.0-6.0ng/ml were prepared. The assay item for digitoxin was used for deslanoside after modification of the parameters. The coefficients of variation of within-run assay and between-run assay in this method were less than 9% at various concentrations tested. By use of a ultrafiltration method, the protein binding percent of deslanoside was determined to be zero or very small. In a patient with renal and hepatic failures, the half life (t1/2) and the apparent volume of distribution (Vd) were calculated to be 131 hours and 4.1l/kg after intravenous administration of deslanoside, respectively. On the other hand, an equation of Y=1.25X+0.08 was obtained from a correlation between digoxin concentration (X) given by normal digoxin assay and concentration of deslanoside (Y). Therefore, with a simple method, one can calculate the deslanoside concentrations.
日本医療薬学会の論文
1993-02-20