ナワシログミに共生する窒素固定菌フランキアの純粋分離

概要

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The purpose of this experiment is to identify microorganisms isolated from Elaeagnus pungens nodules, and to establish a simple estimation method on the effectiveness of inoculation. 1.The mycelia of strains isolated from Elaeagnus pungens nodules had branches with no aerial mycelium in liquid culture conditions, and they formed strawberry-shaped or short cylindrical sporangia containing scores of spores. 2.Inoculation of the Actinomycete strains to cutting branches of Elaeagnus pungens (about 10cm in length) previously rooted and cultured in a test tube was useful as a simple estimation method on nodulation and nitrogen fixation among isolated strains. Moreover, it was found that pretreatment of the cutting branch with kinetin, IBA and NAA was effective for the evaluation of rooting. 3.The cutting branch inoculated with strains isolated from Elaeagnus pungens nodules formed root nodules with nitrogen fixing activity (acethylene reducing activity) and showed favorable growth under the N-free culture condition 4.From these results, strains isolated from Elaeagnus pungens nodules were identified as Frankia belonging to Actinomycetes. Frankia within root nodule cell was histologically observed with an optical microscope.
社団法人日本土壌肥料学会の論文
1995-10-05