Characterization of Cadmium-Tolerant Carrot Cells in Response to Cadmium Stress

概要

論文の詳細を見る
Non-tolerant (CW) and Cd-tolerant (CCd1) carrot (Daucus carota L.) cells were compared for their response to cadmium (Cd). The CCd1 cells were induced by selection from the CW cells on a B5 medium containing 100 μM Cd. The CCd1 cells were more tolerant to Cd than the CW cells. In an 80 μM Cd treatment, the growth rate (8 d/0 d) based on the fresh weight of the CCd1 cells and CW cells was 69 and 24%, respectively, compared to the controls. Cd stress led to the decrease of the total glutathione content and to the increase of the contents of non-protein thiols (t-SH, phytochelatins) and Cd in both types of cells. Under Cd stress, the contents of t-SH and Cd of the CW cells were higher than those of the CCd1 cells. The CW cells in the 80 and 160 μM Cd treatments accumulated Cd with the increase in time. In contrast, the Cdc ontent in the CCd1 cells in all the treatments and in the CW cells in the 40 μM Cd treatment gradually decreased after 1 d. In an additional experiment, both types of cells pretreated with 20-80 μM Cd for 1 d were transferred to a Cd-free medium. The CCd1 cells in all the pretreatments continuously excluded Cd over a 4-dperiod, unlike the CW cells. Therefore, a larger depletion of Cd was found in the CCd1 cells, which may be partially attributed to Cd tolerance.
社団法人日本土壌肥料学会の論文