トランスジェニックマウス gpt delta を用いた点突然変異と欠失突然変異の検出 : 第28回大会シンポジウム : II.新しい変異原検出法へのアプローチ

概要

論文の詳細を見る
A novel transgenic mouse, named gpt delta, has been developed for the efficient detection of mutations in vivo. A feature of the assay system is the incorporation of two distinct positive selections for the detection of two different types of mutations, i.e., point mutations and deletions. The first selection is 6-thioguanine selection, which uses the gpt gene of E. coli as a reporter and mainly detects point mutations such as base substitutions and frameshifts. Since the coding region of the gpt gene is 456 base pairs (bp) , it is convenient for the identification of mutations by DNA sequencing. The second selection is Spi^- selection, which uses the red/gam genes of λ phage as a reporter and preferentially detects deletion mutations with the molecular sizes from 1 bp to about 10,000 bp. The results of the mutation assay with ethylnitrosourea and gamma-ray irradiation are presented.
2000-07-31