阻害剤処理した葉の老化におけるタンパク質およびアミノ酸含量の変化

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The changes in chlorophyll, protein and amino acid were investigated in order to determine the effect of difference of the period treated with the inhibitors of protein and nucleic acid synthesis on senescence of leaf discs which punched from primary leaves of 10 days old bean seedlings (phaseolus vulgaris L.) The chlorophyll contents in leaf discs floated on water (control) and inhibitor solutions for 5 days decreased, and this decreased degree was highest in CAP followed by 1-Ethi, 2,6-DP, 2-TU, control and CH in that order. The daily decrease in chlorophyll during leaf discs senescence promoted in CAP and 2,6-DP treatment, and retarded in CH treatment. This promoting and retarding effects were always larger in discs treated with inhibitor for initial 2 days after the experiment start. Both water soluble protein and thermic noncoagulated protein in leaf discs decreased daily during senescence, and this decreasing tendency was remarkably larger in the former than in the latter. Daily decrease in both proteins accelerated in CAP and 2,6-DP treatment, and prevented in CH treatment, and those accelerating or preventing effects was larger in early inhibitor treatment than in late one. Amino acid in leaf discs increased daily during senescence, and this increased degree was highest in CAP treatment followed by 2,6-DP, control and CH in that order, and was larger in early inhibitor treatment with. From the results, it may be concluded that daily increase of the degradation of chlorophyll and proteins, and the transformation to amino acid is stimulate in CAP and 2,6-DP, and delay in CH, and that those tendency is particularly high in early inhibitor treatments.
福岡女子大学の論文
1989-01-25

阻害剤処理した葉の老化におけるタンパク質およびアミノ酸含量の変化

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