フローサイトメトリーによるフィルター処理血液製剤中の残存白血球数の測定

概要

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私達はフローサイトメトリー(FCM)を用い、フィルター法により作製した白血球除去血液製剤中の微量白血球の測定法の確立を試みた。We detected a very small number of leukocytes in blood products which couldn't be counted with electronic haemocyte counter by the method of flowcytometry and nuclear staining with propidium iodide. This method revealed that the number of residual leukocyte was on the level of 10 in a concentrated red cell product, in the levelof 10 in an washed red cell products and on the level of 10 in a filtered red cell product. In case of platelet products, the number of residual leukocyte was on the level of 10 in a concentrated platelet product and on the level of 10 in a filtered platelet product. Residual leukocytes in filtered blood products fell below the level of 5x10 which was critical number of alloimmunity induction. Blood preservation for 7 days didn't improved filtration rate of leukocytes. Filtration made leukocyte fractions relatively Iymphopenic and granulocytotic in comparison with ones in original products. We expect that improvement of filtration capacity to remove granulocytes will make the number of residual leukocytes fall on the level of 10, which our method can fully deal with.
熊本大学の論文
1995-03-01