フローサイトメトリーによるリンパ球の細胞内pH測定 : 月経周期およびT細胞亜群との関係について

概要

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本研究ではリンパ球を用いてFCMによるpHi測定法の確立のための基礎的条件について検討し、次いで正常リンパ球のpHi値について検討した。We established the optimal condition of intracellular pH measurement of lymphocytes with fluorescent pH indicator by flowcytometry. Mean value with 1SD of pHi in peripheral blood lymphocytes of healthy persons was 7.01±0.12(7.00 ±0.07 in male and 7.02 ±0.16 in female). In case of female, pHi became higher in follicular stage but lower in progestational stage than mean value in whole menstrual cycle. And change of pHi in a menstrual cycle was correlated positively with activated T cell subset and cytotoxic effector T cell subset, but negatively with suppressor effector T cell subset.
熊本大学の論文
1994-01-31