<原著>酸性フォスファターゼ活性とコラーゲン線維の線維芽細胞内消化

概要

論文の詳細を見る
Human gingival fibroblast cell line was cultured with bovine collagen fibrils. The relationship between acid phosphatase activity and phagocytosis and intracellular degradation of collagen by fibroblasts was investigated by cytological and cytochemical methods. Cell processes extended and surrounded collagen fibrils at 1 hr of cultivation: The fibrils were then interiorized in cell processes and ultimately became enclosed within phagosomes. Phagocytosis of collagen induced acid phosphatase activity on fibroblast GERL consisting of tubular and vesicular structures. There was no evidence of an enzymatic lysis of the fibrils in the extracellular space. Primary lysosomes derived from GERL fused with the collagen fibril-containing phagosomes to form phagolysosomes. Collagen degradation occurred within them. The present study demonstrates that the lysosomal system acts as an important part in the intracellular degradation which may play an essential role in the physiological turnover and resorption of collagen.
北海道医療大学の論文
1982-12-31