スルファモノメトキシン・オルメトプリム合剤のヒラメエドワジエラ症に対する治療効果

概要

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Therapeutic effect of a 3:1 combination of sulfamonomethoxine and ormetoprim (SMM-OMP) against edwardsiellosis was studied in two experiments with artificially infected Japanese flounder Paralichthys olivaceus. Flounder, average weight of 25.8±4.8g in experiment 1 and 38.4±7.2 g in experiment 2, were challenged by immersing for 15 min in sea water containing about 108 colony forming unit (CFU)/ml of Edwardsiella tarda NUF251. Slurry made up of ground commercial pellet feed and water containing SMM-OMP was administrated orally into the stomach of experimental fish with a plastic cathetel for 7 days in experiment 1 or 5 days in experiment 2 from the following day of challenge. Dosages of the drug containing 30 mg of SMM and 10 mg of OMP per 100 mg were 0, 25, 50,100,200 and 400 mg/kg/day in experiment 1 and 0, 12.5, 25 and 50 mg/kg/day in experiment 2. Therapeutic effect of oxytetracycline (OTC) was also studied at a dose of 50 mg/kg/day in experiment 2.As a result high therapeutic effects of SMM-OMP were achieved even at the lowest dosage (12.5 mg/kg). In experiment 1, however, the fish at higher dosages were died more than those at lower dosages. Such fish did not show the disease signs of edwardsiellosis, and also E. tarda was not reisolated from the fish. From the results such deaths may be due to a harmful effect of the drug. OTC was proved to be ineffective though E. tarda NUF251 was sensitive to OTC in vitro.