<原著>Neuraminidase処理Colon 26結腸癌細胞の門脈内投与による肝転移モデルの作製

概要

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In this study, a liver metastasis model was established in mice. The in vitro colon 26 cultured cell line was made from an in vivo transplantable colon 26 adenocarcinoma tumor mass by using standard enzymatic treatments (collagenase, DNase). In vitro cultured cells were injected into the portal vein of BALB/c mice for inducing liver metastases after the cells were desialyzed with neuraminidase to promote their uptake into liver cells. Three weeks later, metastatic foci with nontreated colon 26 were found in 50-70% of the mice. By contrast, treated colon 26 cells markedly increased the incidence of hepatic matastases ; countable hapatic colonies were found in all mice (100%). We also confirmed that intravenous injection of galactocerebroside (a competitive inhibitor of D-galactose receptor), prior to intraportal injection, suppressed the liver metastasis of cancer. This results may be related to the liver-characteristic D-galactose receptors since pre-injection with an excess amount of galactocerebroside completely prevented tumor colonization in the liver. This experimental model was found to be useful for the analysis of hepatic metastases from colorectal cancer.
近畿大学の論文
1992-06-25