腸内細菌科の鑑別をかねた新しい確認培地の研究

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Proteus, Morganella, Rettgerella and Providencia act on dl-phenyl alanine and produce phenyl pyruvic acid. (PPA). From the culture filtrate of above microorganisms, crystalline phenyl pyruvic acid was detected. The authors intended to utilize this PPA production of the microorganisms above described for the differentiation of the family Enterobacteriaceae and investigated into the composition of a new medium. The iron which participates in PPA-reaction is a ferric salt. A ferrous salt has no effect on it. From results obtained, a new confirmatory medium which can also serve as differential medium was deviced. By the use of this medium it is possible to observe PPA-reaction, acid and gas production by fermentation of glucose or lactose and hydrogen sulphide production simultaneously. Proteus, Morganella, Rettgerella and Providencia produce a dark red-purple color on the slant surface of this medium. Hence, the differentiation of Salmonella and Shigella from Proteus, Morganella, Rettgerella or Providencia which was impossible on Kligler's medium became possible. Use of this new medium alone, therefore, will enable confirmation and differentiation of the family Enterobacteriaceae and it is considered useful as a routine clinical test.
1964-08-20