Isolation of a Parainfluenza Virus Type 3-like Agent from Guinea Pigs

概要

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Guinea pig (GP) sera seropositive to human parainfluenza virus type 3 (PIV-3) antigen were found previously in our laboratory. In the present study, we attempted to isolate and characterize a new agent from GP colonies. The new agent was isolated from lung homogenate of sentinel GPs, which were maintained in the GP room containing the serologically positive GP breeding colony. This new agent was found to be comparable to human and bovine PIV-3 strains in regard to its moderate cytopathic effects, hemagglutinating activity, and neutralizing activity, and moreover, it was found to produce antigens cross-reactive to both of the latter PIV-3s. The resemblance between the new isolates and the other PIV-3s was supported by the SDS-PAGE and Western blot analysis of the viral proteins. This is the first report of the isolation of a type 3-like parainfluenza virus from GP. This finding is significant, insofar as an inapparent infection with the virus may concern animal experimentation in the use of GP.
長崎大学の論文