Anti-Macrophage Migration Inhibitory Factor Antibody Suppresses Chronic Rejection of Heterotopically Transplanted Trachea in Rats
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概要
- 論文の詳細を見る
Macrophage migration inhibitory factor (MIF) is a pro-inflammatory cytokine essential for delayed hypersensitivity in vivo, and is involved in bronchiolitis obliterans and late rejection in lung transplantation. We tested here whether neutralization of MIF using anti-MIF antibody prevents such a response. We examined the MIF mRNA expression level and changes in allograft tracheal epithelium and intraluminal obstruction in a rat allograft model. Lewis rat (RT1l) underwent heterotopic tracheal transplantation from Brown Norway rats (RT1n) in the omentum. Anti-MIF antibody was injected in the peritoneum. Rats were divided into three groups (non-treated allograft, allograft treated with normal rabbit IgG and allograft treated with anti-MIF antibody). Implants were harvested on days 7 or 21 for histological analysis. MIF mRNA expression was higher in the allograft at days 7 and 21 than in the isograft. The epithelium in non-treated allograft was almost absent at day 7. The epithelial height in the anti-MIF-treated graft was higher than that in normal IgG-treated grafts. The intraluminal space was mostly replaced by granulation tissue at day 21 in the untreated group. The proportion of obliterans was lowest in the anti-MIF group, the second lowest in the untreated group and the third lowest in the normal IgG-treated grafts, and the difference was significant (p<0.001) between the first two groups. Our results indicate that anti-MIF antibody suppresses allogenic tracheal rejection.
- 長崎大学の論文
著者
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大澤 一貴
長崎大学先導生命科学研究支援センター
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SUMIDA YORIHISA
Division of Surgical Oncology, Department of Translational Medical Sciences, Nagasaki University Gra
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Sumida Yorihisa
Division Of Surgical Oncology And Department Of Translational Medical Sciences Nagasaki University S
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Sumida Yorihisa
Division Of Surgical Oncology Nagasaki University Graduate School Of Biomedical Sciences
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Ohsawa Kazutaka
Biomedical Research Center for Frontier Life Sciences, Nagasaki University Graduate School of Biomed
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大沢 一貴
長崎大学先導生命科学研究支援センター・比較動物医学分野
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Ohsawa Kazutaka
Biomedical Research Center For Frontier Life Sciences Nagasaki University Graduate School Of Biomedi
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Ohsawa Kazutaka
Laboratory Animal Center For Biomedical Research Nagasaki University School Of Medicine
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Ohsawa Kazutaka
Div. Of Comparative Medicine Center For Frontier Life Sciences Nagasaki Univ.
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Yamashita Hideki
Biomedical Research Center for Frontier Life Sciences, Nagasaki University Graduate School of Biomedical Sciences
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