Biochemical studies of Antarctic algae: Detection of ferredoxin by high performance liquid chromatography

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Two clones of Chlorella (A1 and A2) were isolated from an algal mat collected from a fresh-water pond, Lake Yukidori, Langhovde, and were cultured in the laboratory. Ferredoxins were isolated from these Chlorella cells and analyzed by high performance liquid chromatography (HPLC) using a TSK-gel Phenyl-5PW column for hydrophobic chromatography and a DEAE-5PW column for ion-exchange chromatography. Chlorella A1 cells contained two distinct ferredoxins separable by hydrophobic but not by ion-exchange chromatography. Chlorella A2 cells also contained two distinct ferredoxins whose retention times in the hydrophobic HPLC analysis were different from these of ferredoxins in A1 cells. Ferredoxins were also isolated directly from an algal mat collected at another fresh-water pond, Lake Skallen Oike, Skallen. Cyanobacterial and non-cyanobacterial ferredoxins were separately extracted from the algal mat. Cyanobacterial ferredoxins extracted by sonication of the mat in 35% saturated ammonium sulfate were separated into four peaks by the hydrophobic chromatography. Non-cyanobacterial ferredoxins, extracted from acetone powder of the mat debris remaining after extraction of cyanobacterial ferredoxins, were separated into two peaks. This result suggests that the algal mat contains at least two cyanobacterial and one non-cyanobacterial species.
国立極地研究所の論文