<Originals>An Accurate Microassay for Interferon : Its Use for Evaluation of Interferon Activity Produced in Lymphocyte Cultures Stimulated with PHA, PPD and Thyroglobulin
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概要
- 論文の詳細を見る
A convenient microassay for interferon as a variation of radiochemical methods was developed in this laboratory. The assay was evaluated to be excellent in speed, economy and reproducibility as well as capability of handling a large number of samples in each assay run, indicating a great utility for clinical researches. By utilizing the present assay, production of mitogen or antigen-induced interferons was studied for lymphocyte cultures. Different types of kinetics were obtained for interferon production between phytohemagglutinin (PHA) and antigen-stimulated cultures : the peak interferon production was demonstrated between 3-4 days and after 7 days in each culture driven with PHA and purified tuberculoprotein derivatives (PPD) respectively. The study of thyroglobulin (Tg)-induced interferon production was also performed for lymphocytes of various origins and disclosed that the positive response was limited to the patients with thyroid diseases. Lymphocytes obtained from a patient with chronic lymphocytic leukemia with marked T cell dysfunctions never produced either PPD or Tg-induced interferon in vitro. Because Tg-induced interferon production was noticed for lymphocyte cultures of a patient with subacute thyroiditis, the ability was serially examined in conjunction with the measurement of Tg levels in serum for over 10 months. The producibility of Tg-induced interferon was just transiently positive for a while when peripheral Tg levels of the patient remained abnormally elevated and revealed as negative after 9 months from the onset of the disease when Tg levels in serum were normalized. It was postulated that sustained elevation of Tg in circulation might first cause the expansion of autoreactive T cells, directed to Tg, causing appreciable cell-mediated immunity to the protein, the event presumably leading to the development of anti-Tg.
- 近畿大学の論文
- 1981-06-28
著者
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Aoki Norihiko
Second Department Of Internal Medicine Kinki University School Of Medicine
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HARA Mukuyu
Kansaidenryoku Hospital
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AOKI Norihiko
Second Department of Internal Medicine, Kinki University School of Medicine
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- An Accurate Microassay for Interferon : Its Use for Evaluation of Interferon Activity Produced in Lymphocyte Cultures Stimulated with PHA, PPD and Thyroglobulin