Identification and Evolution of Venom Phospholipase A_2 Inhibitors from Protobothrops elegans Serum

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概要

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• The cDNAs encoding venom phospholipase A2 (PLA2) inhibitors (PLIs), named Protobothrops elegans (Pe)γPLI-A, PeγPLI-B, PeαPLI-A, and PeαPLI-B, were cloned from the P. elegans liver cDNA library. They were further divided into several constituents due to nucleotide substitutions in their open reading frames. For PeαPLI-A, two constituents, PeαPLI-Aa and PeαPLI-Ab, were identified due to three nonsynonymous substitutions in exon 3. Far-western blot and mass-spectrometry analysis of the P. elegans serum proteins showed the presence of γPLIs, and αPLIs, which can bind venom PLA2s. In αPLIs from Protobothrops sera, A or B subtype-specific amino acid substitutions are concentrated only in exon 3. A comparison of γPLIs showed that γPLI-As are conserved and γPLI-Bs diversified. Mathematical analysis of the nucleotide sequences of Protobothrops γPLI-B cDNAs revealed that the particular loops in the three-finger motifs diversified by accelerated evolution. Such evolutionary features should have made serum PLIs acquire their respective inhibitory activities to adapt to venom PLA2 isozymes.

• 社団法人 日本農芸化学会の論文
• 2011-03-23

著者

• 倉石 武

  東京大学大学院農学生命科学研究科

• OHNO Motonori

  Department of Applied Microbial Technology, Kumamoto Institute of Technology

• 倉石 武

  東京大学医科学研究所

• Ikeda N

  National Institute For Materials Science

• Murakami Tatsuo

  Department Of Anesthesia Gifu Red Cross Hospital

• CHIJIWA Takahito

  Department of Applied Life Science, Faculty of Bioscience and Biotechnology, Sojo University

• SO Shuhei

  Department of Applied Life Science, Faculty of Bioscience and Biotechnology, Sojo University

• KURAISHI Takeshi

  Institute of Medical Science, The University of Tokyo

• HATTORI Shosaku

  Institute of Medical Science, The University of Tokyo

• Ikeda Naoki

  Department Of Applied Life Science Faculty Of Bioscience And Biotechnology Sojo University

• ODA UEDA

  Department of Biochemistry, Faculty of Pharmaceutical Sciences, Sojo University

• Oda Ueda

  Department Of Biochemistry Faculty Of Pharmaceutical Sciences Sojo University

• So Shuhei

  Department Of Applied Life Science Faculty Of Bioscience And Biotechnology Sojo University

• Hattori Shosaku

  Institute Of Medical Science The University Of Tokyo

• Murakami Tatsuo

  Department Of Applied Life Science Faculty Of Bioscience And Biotechnology Sojo University

• Chijiwa Takahito

  Department Of Applied Life Science Faculty Of Bioscience And Biotechnology Sojo University

• Kuraishi Takeshi

  Institute Of Medical Science The University Of Tokyo

• Ohno Motonori

  Department Of Applied Life Science Faculty Of Bioscience And Biotechnology Sojo University

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