A [Lys^<49>]phospholipase A_2 from Protobothrops flavoviridis Venom Induces Caspase-Independent Apoptotic Cell Death Accompanied by Rapid Plasma-Membrane Rupture in Human Leukemia Cells

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概要

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• Protobothrops flavoviridis venom contains plural phospholipase A2 (PLA2) isozymes. A [Lys49]PLA2 called BPII induced cell death in human leukemia cells. PLA2, an [Asp49]PLA2 that has much stronger lipolytic activity than BPII, failed to induce cell death. BPII-treated cells showed morphological changes, DNA fragmentation, and nuclear condensation. This BPII-induced apoptotic cell death was neither inhibited by inhibitors of caspases 3 and 6 nor accompanied by activation of procaspase 3, indicating that BPII-induced cell death is caspase independent. Since inactive p-bromophenacylated BPII induced cell death, BPII-induced apoptotic cell death is independent of PLA2 lipolytic activity. Rapid externalization of phosphatidylserine in BPII-treated cells was observed for fluorescein isothiocyanate (FITC)-labeled annexin V. In the cells treated with BPII, this spread over the cell membranes, implying that the cell toxicity of BPII is mediated via its cell-surface receptor.

• 2011-05-23

著者

• OHNO Motonori

  Department of Applied Microbial Technology, Kumamoto Institute of Technology

• Murakami Tatsuo

  Department Of Anesthesia Gifu Red Cross Hospital

• KAMIKADO Nobuyuki

  Department of Applied Microbial Technology, Faculty of Bioscience and Biotechnology, Sojo University

• FUJIMOTO Ryo

  Department of Applied Microbial Technology, Faculty of Bioscience and Biotechnology, Sojo University

• HAMAGUCHI Kazuhiko

  Department of Applied Microbial Technology, Faculty of Bioscience and Biotechnology, Sojo University

• CHIJIWA Takahito

  Department of Applied Life Science, Faculty of Bioscience and Biotechnology, Sojo University

• Nakamura Hitomi

  Laboratory Of Biological Chemistry Department Of Pharmaceutical Sciences Faculty Of Pharmaceutical S

• ODA UEDA

  Department of Biochemistry, Faculty of Pharmaceutical Sciences, Sojo University

• Oda Ueda

  Department Of Biochemistry Faculty Of Pharmaceutical Sciences Sojo University

• Fujimoto Ryo

  Department Of Applied Microbial Technology Faculty Of Bioscience And Biotechnology Sojo University

• ODA UEDA

  Laboratory of Biological Chemistry, Department of Pharmaceutical Sciences, Faculty of Pharmaceutical

• Murakami Tatsuo

  Department Of Applied Life Science Faculty Of Bioscience And Biotechnology Sojo University

• Chijiwa Takahito

  Department Of Applied Life Science Faculty Of Bioscience And Biotechnology Sojo University

• Kamikado Nobuyuki

  Department Of Applied Microbial Technology Faculty Of Bioscience And Biotechnology Sojo University

• Hamaguchi Kazuhiko

  Department Of Applied Microbial Technology Faculty Of Bioscience And Biotechnology Sojo University

• Ohno Motonori

  Department Of Applied Life Science Faculty Of Bioscience And Biotechnology Sojo University

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