Chemical Studies on Different Color Development in Blue- and Red-Colored Sepal Cells of Hydrangea macrophylla
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概要
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To clarify the cause of the difference in blue and red color development of hydrangea sepals, Hydrangea macrophylla, we analyzed the organic and inorganic components in the colored cells. To obtain colored protoplasts, each blue and red sepal tissue was treated with a combination of cellulase and pectinase, and then from the suspension of the olored and colorless protoplast mixture colored cells of the same hue were collected with a micro-pipette. The content of organic components (delphinidin 3-glucoside, chlorogenic acid, neochlorogenic acid and 5-O-p-coumaroylquinic acid) and Al3+ in each colored cell was quantified respectively by semimicro-HPLC and graphite furnace atomic absorption spectroscopy (GFAAS). In the blue cells 13 eq. of 5-O-acylquinic acids and 1.2 eq. of Al3+ to anthocyanin were contained. Contrary to this result, in the red cells, only 3.6 eq. of 5-O-acylquinic acids and 0.03 eq. of Al3+ were detected. A reproduction experiment of each blue and red sepal color by mixing those components concluded that, for blue coloration, both 5-O-acylquinic acids and Al3+ were essential.
- 社団法人 日本農芸化学会の論文
- 2009-05-23
著者
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YOSHIDA Kumi
Graduate School of Information Science, Nagoya University
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KONDO Tadao
Graduate School of Bioagricultural Sciences, Nagoya University
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Ito Daisuke
Graduate School of Engineering, Hokkaido Universuty Sapporo Japan
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Kondo Tadao
Graduate School Of Information Sci. Nagoya Univ.
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Kondo Tadao
Graduate School Of Bioagricultural Sciences Nagoya University
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Yoshida Kumi
Graduate School Of Information Science Nagoya University
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Yoshida Kumi
Graduate School Of Information Sciences Nagoya University
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KATO YUKI
Graduate School of Information Science, Nara Institute of Science and Technology
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SHINKAI Yosuke
Graduate School of Human Informatics, Nagoya University
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Shinkai Yosuke
Graduate School Of Human Informatics Nagoya University
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Kato Yuki
Graduate School Of Information Science Nagoya University
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Ito Daisuke
Graduate School Of Information Science Nagoya University
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Kato Yuki
Graduate School Of Information Science And Technology Hokkaido University
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