Cloning and characterization of ACC oxidase genes from tulip
スポンサーリンク
概要
- 論文の詳細を見る
Five cDNA clones encoding 1-aminocyclopropane-1-carboxylic acid (ACC) oxidase (ACO) were isolated from tulip (Tulipa gesneriana L.) by differential screening of the petal cDNA library and were designated as TgACO1, TgACO2, TgACO3, TgACO4 and TgACO5. The deduced amino acid sequences exhibited similarity to ACO proteins from other plant species. Among these proteins, TgACO1-4 have high similarity (90–94% identity) each other, whereas TgACO5 showed low similarity compared with the other four proteins (50% identity). Phylogenic analysis indicated that TgACO1-4 and TgACO5 are distant. Genomic analysis of TgACO1-4 showed that they are organized into four exons and three introns, whereas TgACO5 consists of three exons interrupted by two introns. Real-time RT-PCR analysis of gene expression revealed that TgACO1, TgACO3 and TgACO5 were expressed in wilting petals, leaves, and stems, respectively, whereas TgACO2 and TgACO4 were expressed only at basal levels in these tissues. Therefore, tulip ACO genes seem to be regulated differentially among the vegetative tissues and during flower senescence.
- 日本植物細胞分子生物学会の論文
- 2007-03-01
著者
-
Yoshida Kumi
Graduate School Of Information Science Nagoya University
-
Shoji Kazuaki
Agricultural Experiment Station Toyama Agricultural Research Center
-
Momonoi Kazumi
Agricultural Experiment Station, Toyama Agricultural Research Center
関連論文
- The Involvement of Tonoplast Proton Pumps and Na^+(K^+)/H^+ Exchangers in the Change of Petal Color During Flower Opening of Morning Glory, Ipomoea tricolor cv. Heavenly Blue
- Sepal Color Variation of Hydrangea macrophylla and Vacuolar pH Measured with a Pronton-Selective Microelectrode
- Synchrony between flower opening and petal-color change from red to blue in morning glory, Ipomoea tricolor cv. Heavenly Blue
- P-71 BLUE FLOWER COLOR DEVELOPMENT OF THE HIMALAYAN BLUE POPPY
- P-377 Synthesis of Cyanidin 3-O-β-D-Glucoside
- Chemical Studies on Different Color Development in Blue- and Red-Colored Sepal Cells of Hydrangea macrophylla
- Alternative Expression of Vacuolar Iron Transporter and Ferritin Genes Leads to Blue/Purple Coloration of Flowers in Tulip cv. 'Murasakizuisho'
- Anthocyanin Components and Mechanism for Color Development in Blue Veronica Flowers
- P-356 The Mechanism Underlying Blue Color Development in Tulips
- Perianth Bottom-Specific Blue Color Development in Tulip cv. Murasakizuisho Requires Ferric Ions
- P-111 Studies on Color Variation of Hydrangea macrophylla on the Basis of Single Cell Analysis
- P-581 Structural elucidation of chiral arrangement among flavocommelin molecules involving in blue flower color development by using of 6-31G(d)
- Cloning and characterization of ACC oxidase genes from tulip
- Urakunoside, a New Tetraglycosyl Kaempferol from Petals of the Wabisuke Camellia cv. Tarokaja
- Urakunoside, a New Tetraglycosyl Kaempferol from Petals of the Wabisuke Camellia cv. Tarokaja
- Specific Expression of the Vacuolar Iron Transporter, TgVit, Causes Iron Accumulation in Blue-Colored Inner Bottom Segments of Various Tulip Petals