Expression of a Functional Sphingomyelinase of Pseudomonas sp. TK4 in Mammalian Cells
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概要
- 論文の詳細を見る
We report here the expression of a bacterial sphingomyelinase in mammalian cells as a functionally active form. A chimeric Pseudomonas sphingomyelinase fused with the lysosomal sorting motif of lysosomal acid phosphatase was sorted to lysosomes in mammalian cells. As expected, the chimeric SMase hydrolyzed sphingomyelin in vivo to produce ceramide, part of which was converted to glucosylceramide.
- 社団法人 日本農芸化学会の論文
- 2007-02-23
著者
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Ito Makoto
Department Of Cardiovascular And Respiratory Medicine Shiga University Of Medical Science
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Sueyoshi Noriyuki
Department Of Bioscience And Biotechnology Graduate School Of Bioresource And Bioenvironmental Scien
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Sueyoshi Noriyuki
Dep. Of Bioscience And Biotechnology Graduate School Of Bioresource And Bioenvironmental Sciences Ky
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Horibata Yasuhiro
Department Of Bioscience And Biotechnology Graduate School Of Bioresource And Bioenvironmental Scien
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Ito Makoto
Dep. Of Bioscience And Biotechnology Graduate School Of Bioresource And Bioenvironmental Sciences Ky
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Ito Makoto
Department Of Bioscience And Biotechnology Graduate School Of Bioresource And Bioenvironmental Scien
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ITO Makoto
Department of Bioresources, Faculty of Agriculture, Ehime University
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