Cloning and Expression of Gene Encoding a Novel Endoglycoceramidase of Rhodococcus sp. Strain C9.

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概要

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• Endoglycoceramidase (EGCase) is an enzyme capable of cleaving the glycosidic linkage between oligosaccharides and ceramides of various glycosphingolipids. We previously reported that the Asn-Glu-Pro (NEP) sequence is part of the active site of EGCase of Rhodococcus sp. strain M-777. This paper describes the molecular cloning of a new EGCase gene utilizing the NEP sequence from the genomic library of Rhodococcus sp. strain C9, which was clearly distinguishable from M-777 by 16S rDNA analysis. C9 EGCase possessed an open reading frame of 1, 446 by encoding 482 amino acids, and showed 78% and 76% identity to M-777 EGCase II at the nucleotide and amino acid levels, respectively. Interestingly, C9 EGCase showed the different specificity to the M-777 enzyme: it hydrolyzed b-series gangliotetraosylceramides more slowly than the M-777 enzyme, whereas both enzymes hydrolyzed a-series gangliosides and neutral glycosphingolipids to the same extent.

• 社団法人 日本生化学会の論文

著者

• Sueyoshi Noriyuki

  Department Of Bioscience And Biotechnology Graduate School Of Bioresource And Bioenvironmental Scien

• Okino Nozomu

  Department Of Bioscience And Biotechnology Graduate School Of Bioresource And Bioenvironmental Scien

• Izu Hiroyuki

  Biotechnology Research Laboratories Takara Shuzo Co.

• ITO Makoto

  Department of Bioresources, Faculty of Agriculture, Ehime University

• Sakaguchi Keishi

  Department of Bioscience and Biochnology, Graduate School of Bioresource and Bioresource and Bioenvironmental Sciences, School Kyushu University

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