Glimepiride Treatment Upon Reperfusion Limits Infarct Size via the Phosphatidylinositol 3-Kinase/Akt Pathway in Rabbit Hearts
スポンサーリンク
概要
- 論文の詳細を見る
The phenomenon termed postconditioning, that is, brief episodes of ischemia/reperfusion at the onset of reperfusion reduce infarct size, is thought to involve the activation of the phosphatidylinositol 3-kinase (PI3K)/Akt pathway. Treatment with a drug activating PI3K at the onset of reperfusion may confer a similar cardioprotection. The sulfonylurea glimepiride has been shown to activate PI3K in human endothelial cells. We therefore tested in rabbit hearts whether glimepiride can produce postconditioning-mimetic actions. Langendorff-perfused rabbit hearts were subjected to 30 min of global ischemia and 120 min of reperfusion, and infarct size was determined by triphenyltetrazolium staining. Phosphorylation of Akt was analyzed by Western blotting. Glimepiride (10 μM) treatment for the first 10 min of reperfusion significantly reduced infarct size from 67.2 ± 1.3% in controls to 35.8 ± 4.5% (P<0.01). This infarct size–limiting effect of glimepiride was abolished by a selective inhibitor of PI3K (5 μM LY294002, 65.4 ± 3.4%). Phosphorylation of the PI3K substrate Akt was significantly increased in glimepiride-treated hearts when compared to controls (P<0.05). Glimepiride-induced Akt phosphorylation was inhibited by LY294002. In conclusion, our study demonstrates that glimepiride treatment upon reperfusion reduces infarct size in rabbit hearts via a PI3K/Akt-mediated pathway. The postconditioning-mimetic action of glimepiride may be beneficial for the treatment of diabetic patients with ischemic heart disease.
- 2009-02-20
著者
-
NISHIDA Hirofumi
Department of Pharmacology, Chiba University Graduate School of Medicine
-
SATO Toshiaki
Department of Pharmacology, Chiba University Graduate School of Medicine
-
NOMURA Momoko
Department of Pharmacology, Chiba University Graduate School of Medicine
-
MIYAZAKI Masaru
Department of General Surgery, Chiba University Graduate School of Medicine
-
NAKAYA Haruaki
Department of Pharmacology, Chiba University Graduate School of Medicine
-
Nakaya Haruaki
千葉大学 医学研究院薬理学
-
Nomura Momoko
Department Of Pharmacology Chiba University Graduate School Of Medicine
-
Nakaya Haruaki
Department Of Orthopaedic Surgery And Pharmacology School Of Medicine Chiba University
-
Miyazaki Masaru
Department Of General Surgery Chiba University Graduate School Of Medicine
-
Sato Toshiaki
Department Of Applied Physics Faculty Of Science Science University Of Tokyo
-
Nishida Hirofumi
Department Of Pharmacology Chiba University Graduate School Of Medicine
-
Nishida Hirofumi
Department Of Cardiovascular Surgery Chiba University Hospital
-
Miyazaki Masaru
Department Of General Surgery Chiba University
関連論文
- Glimepiride Treatment Upon Reperfusion Limits Infarct Size via the Phosphatidylinositol 3-Kinase/Akt Pathway in Rabbit Hearts
- 27aSA-4 静穏時オーロラの巨視的アーク構造形成における微視的不安定性の効果(27aSA プラズマ宇宙物理(シミュレーション技法),領域2(プラズマ基礎・プラズマ科学・核融合プラズマ・プラズマ宇宙物理))
- FRS-009 Crucial roles of PDK1 in the regulation of β-adrenergic response and cell survival in the hearts(Novel Pathophysiological Mechanisms of Heart Failure(M),Featured Research Session,The 72nd Annual Scientific Meeting of the Japanese Circulation Socie
- OE-056 Erythropoietin Prevents LV Remodeling after Myocardial Infarction through Erythropoietin Receptor-Induced Signaling Pathways in the Heart(Acute myocardial infarction, basic-1, The 71st Annual Scientific Meeting of the Japanese Circulation Society)
- OJ-242 Erythropoietin Has an Ischemic Postconditioning-like Effect on Myocardium through a Direct Action on Cardiomyocytes(Myocardial ischemia-reperfusion, basic/clinical-2 (M) OJ41,Oral Presentation (Japanese),The 70th Anniversary Annual Scientific Meeti
- The Direct Effects of G-CSF on Cardioprotection against Ischemia/Reperfusion Injury(Myocardial Ischemia-reperfusion, Basic 3 (IHD), The 69th Annual Scientific Meeting of the Japanese Circulation Society)
- Optimal Treatment Strategy for Patients With Paroxysmal Atrial Fibrillation : J-RHYTHM Study(Arrhythmia/Electrophysiology)
- New Aspects for the Treatment of Cardiac Diseases Based on the Diversity of Functional Controls on Cardiac Muscles : Mitochondrial Ion Channels and Cardioprotection
- OJ-241 Adrenomedullin Potentiates the Opening of Mitochondrial Ca^-Activated K^+ Channels and Reduces Infarct Size in Rabbit Hearts(Myocardial ischemia-reperfusion, basic/clinical-2 (M) OJ41,Oral Presentation (Japanese),The 70th Anniversary Annual Sci
- OJ-243 Role of c-Src Tyrosine Kinase in Ischemic Myocardium(Myocardial ischemia-reperfusion, basic/clinical-2 (M) OJ41,Oral Presentation (Japanese),The 70th Anniversary Annual Scientific Meeting of the Japanese Circulation Society)
- OJ-241 Adrenomedullin Potentiates the Opening of Mitochondrial Ca^-Activated K^+ Channels and Reduces Infarct Size in Rabbit Hearts(Myocardial ischemia-reperfusion, basic/clinical-2 (M) OJ41,Oral Presentation (Japanese),The 70th Anniversary Annual Sci
- FRS-009 Crucial roles of PDK1 in the regulation of β-adrenergic response and cell survival in the hearts(Novel Pathophysiological Mechanisms of Heart Failure(M),Featured Research Session,The 72nd Annual Scientific Meeting of the Japanese Circulation Socie
- OJ-242 Erythropoietin Has an Ischemic Postconditioning-like Effect on Myocardium through a Direct Action on Cardiomyocytes(Myocardial ischemia-reperfusion, basic/clinical-2 (M) OJ41,Oral Presentation (Japanese),The 70th Anniversary Annual Scientific Meeti
- 3 Clinical Characteristics and Outcomes of Syncope Related to Coronary Spasm(Recent Progress in the Management of Vasospastic Angina,Meet the Expert 6 (ME-6) (IHD),The 73rd Annual Scientific Meeting of The Japanese Circulation Society)
- Effects of Antiarrhythmic Drugs on the Hyperpolarization-Activated Cyclic Nucleotide-Gated Channel Current
- Biliary tract cancer treatment : 5,584 results from the Biliary Tract Cancer Statistics Registry from 1998 to 2004 in Japan
- FRS-069 PDK-1 Plays Essential Roles in Cell Size Regulation, Beta-adrenergic Response and Cell Survival in the Heart(Heart Failure, Cardiac Remodeling and Cardiomyopathy 1, The 71st Annual Scientific Meeting of the Japanese Circulation Society)
- PE-569 Brown Adipose Tissue Derived-cells Differentiate into Spontaneous Beating Cells with both Cardiac Conduction and Pace-maker Characters(Regeneration (angiogenesis/myocardial regeneration)-4, The 71st Annual Scientific Meeting of the Japanese Circula
- Altered deactivation kinetics modify the inhibitory effects of class III antiarrhythmic drugs on HERG channel
- Vasopressin V_1 - Receptor Stimulation Produces a Positive Inotropic Response without Affecting pH_i in Guinea Pig Papillary Muscles
- PIVKA-II-producing advanced gastric cancer
- Evidence for Weak-Ferroelectricity of (NH_4)_2ZnCl_4 in the Phase between Ferro- and Antiferro-Electric Phases
- Stenting and interventional radiology for obstructive jaundice in patients with unresectable biliary tract carcinomas
- Radiation therapy and photodynamic therapy for biliary tract and ampullary carcinomas
- Guidelines for chemotherapy of biliary tract and ampullary carcinomas
- Guidelines for the management of biliary tract and ampullary carcinomas : surgical treatment
- Diagnosis of biliary tract and ampullary carcinomas
- Preoperative biliary drainage for biliary tract and ampullary carcinomas
- Risk factors for biliary tract and ampullary carcinomas and prophylactic surgery for these factors
- Flowcharts for the management of biliary tract and ampullary carcinomas
- Purpose, use, and preparation of clinical practice guidelines for the management of biliary tract and ampullary carcinomas
- PE-484 Limitations on Japanese Localization of a Web-based Remote Monitoring System for Cardiac Pacing Devices : Problems of the Telephone(PE081,Arrhythmia, Others (Clinical/Diagnosis/Treatment) 4 (A),Poster Session (English),The 73rd Annual Scientific Me
- Evaluation of the Risk of Drug-Induced Long QT Syndrome : Electrophysiological Analysis Using Native Cardiac Cells and Cultured Cells Expressing HERG Channels(SEMINAR 1 : MODELS FOR PROFILING THE POTENTIAL QTc PROLONGATION RISK OF A DRUG (ICH-57B)ICH SAFE
- Effects of Azimilide on the Muscarinic Acetylcholine Receptor-Operated K^+ Current and Experimental Atrial Fibrillation in Guinea-Pig Hearts
- Sarcolemmal ATP-sensitive K^+ channels and cardiovascular function
- Effects of Nicorandil on the cAMP-Dependent Cl− Current in Guinea-Pig Ventricular Cells
- Usefulness of intraoperative fluorescence imaging to evaluate local anatomy in hepatobiliary surgery
- FRS-171 Role of Na+-Ca2+ Exchanger in Myocardial Ischemia/Reperfusion Injury : Evaluation Using a Heterozygous Na+-Ca2+ Exchanger Knockout Mouse Model(Coronary Heart Disease : Basic Science (IHD) : FRS20)(Featured Research Session (English))
- Trial of Qualitative Evaluation for Vascular Wall of Abdominal Aortic Aneurysms Using MDCT
- Late-Term Regression of Stenosis at the Kink Site in the Internal Thoracic Artery in Two Cases
- Bypass Surgery with Autologous Peripheral-blood Mononuclear Cells Implantation for Foot Salvage
- Starting Selective Cerebral Perfusion from the Beginning of Cardiopulmonary Bypass to Prevent Cerebral Embolization During Total Arch Replacement
- Heparin-Induced Thrombocytopenia After Coronary Artery Bypass Grafting with Cardiopulmonary Bypass : Report of a Case
- Omental Transfer for Mediastinitis in a Patient with Early Gastric Cancer
- Is It Worthwhile to Perform Infrapopliteal Bypass Surgery for Non-responders of Angiogenesis Therapy with Autologous Peripheral-blood Mononuclear Cells Implantation?
- Effects of lipo-prostaglandin E_1 on blood flow and oxygen pressure in lumbo-sacral nerve roots
- Angiosarcoma Arising in the Breast Following Breast-Conserving Surgery with Radiation for Breast Carcinoma
- Effect of c-fos overexpression on development and proliferation of peritoneal B cells
- Recent advance in the treatment of hilar cholangiocarcinoma : hepatectomy with vascular resection
- X-Ray Diffraction Study on a New Incommensurate Phase Transition in Ferroelectric (NH_4)_2ZnCl_4
- X-Ray Diffraction Study on a New Incommensurate Phase Transition in Ferroelectric (NH_4)_2ZnBr_4
- Breast cancer with cartilaginous and/or osseous metaplasia
- Follow-up of Cases with False-Negative Pathologic Sentinel Nodes in Breast Cancer
- Comedonecrosis is an unfavorable marker in node-negative invasive breast carcinoma
- Dynamic-Enhanced MRI Predicts Metastatic Potential of Invasive Ductal Breast Cancer
- Clinical Implications of Unusual NeuroD and mASH1 Expression in a Patient with Primary Large-Cell Neuroendocrine Carcinoma of the Duodenum : Report of a Case
- CCAAT/enhancer binding protein-β promotes the survival of intravascular rat pancreatic tumor cells via antiapoptotic effects
- Treatment with an oral fluoropyrimidine, S-1, plus cisplatin in patients who failed postoperative gemcitabine treatment for pancreatic cancer : a pilot study
- Right hepatectomy combined with retrohepatic caval resection, using a left renal vein patch graft for advanced cholangiocarcinoma
- Establishment of Gemcitabine-Resistant Human Pancreatic Cancer Cells and Effect of Brefeldin-A on the Resistant Cell Line
- Extended hepatic resection and outcomes in intrahepatic cholangiocarcinoma
- Effects of Bepridil and Amiodarone on Ouabain-Induced Mitochondrial Ca^ Overload in Guinea-Pig Ventricular Cells
- Ischemia-induced electrophysiological alterations in the heart of Kir6.2-deficient mice
- Minoxidil sulfate confers cardioprotection via activation of mitochondrial K_ channels in guinea-pig ventricular myocytes
- ATP-Sensitive K^+ Channel Modulates the Secretion of Atrial Natriuretic Peptide : A Study Using Kir6.2-Deficient Mice (Excitation-contraction Coupling/Ion Channel 2 (A), The 69th Annual Scientific Meeting of the Japanese Circulation Society)
- A Case Complicated with Horner's Syndrome after Off-pump Coronary Artery Bypass
- Surgical Approach to Tumor Thrombus of Renal Cell Carcinoma at the Level between Hepatic Vein and Diaphragm
- Inhibition of ATP-Sensitive K+ Channels and L-Type Ca2+ Channels by Amiodarone Elicits Contradictory Effect on Insulin Secretion in MIN6 Cells
- Quality of guideline development assessed by the Evaluation Committee of the Japan Society of Clinical Oncology
- Tumor reduction rate predicts early recurrence in patients with breast cancer failing to achieve complete response to primary chemotherapy
- Ectopic Breast Tissue in Axillary Lymph Node
- Sentinel Node Micrometastasis and Distant Failure in Breast Cancer Patients
- Prostacyclin Attenuates Oxidative Damage of Adult Rat Myocytes via Opening Mitochondrial ATP-Sensitive Potassium Channels
- A POPULATION STUDY OF LEVELS OF SERUM URIC ACID : The mean value and normal range
- Ischemic preconditioning was mediated by protein kinase C but not phosphatidylinositol-3'-kinase in guinea-pig hearts
- Phyllodes Tumor Arising in Ectopic Breast Tissue of the Axilla
- Immunosuppression Following Surgical and Traumatic Injury
- One hundred seven consecutive surgical resections for hilar cholangiocarcinoma of Bismuth types II, III, IV between 2001 and 2008
- Surgical strategy for mucin-producing bile duct tumor
- Endoscopic diagnosis of intraductal papillary mucinous neoplasm of the bile duct
- Will Subareolar Injection Be a Standard Technique for Sentinel Lymph Node Biopsy?
- Effects of Nicorandil on the cAMP-Dependent Cl^- Current in Guinea-Pig Ventricular Cells
- Effects of GABAergic Drugs on the Recovery of Reflex Potentials after Spinal Cord Ischemia in Cats
- EFFECTS OF VARIOUS DRUGS ON CONDUCTION DELAY INDUCED BY ACUTE MYOCARDIAL ISCHEMIA IN DOGS : Arrhythmia II : IInd Auditorium : Proceedings of the 43rd Annual Meeting of the Japanese Circulation Society, Tokyo, 1979
- QT PRODACT : Database Construction for the Evaluation of the Risk of QT Interval Prolongation by Drugs in Japan
- Impact of nodal involvement on surgical outcomes of intrahepatic cholangiocarcinoma : a multicenter analysis by the Study Group for Hepatic Surgery of the Japanese Society of Hepato-Biliary-Pancreatic Surgery
- PE-357 NS1619 Opens the Mitochondrial Calcium-Activated K^+ Channels and Attenuates the Mitochondrial Ca^ Overload in Guinea-Pig Ventricular Cells(Cardiovascular Pharmacology, Basic/Clinical 4 (H) : PE61)(Poster Session (English))
- Cardioprotective effect of diazoxide is mediated by activation of sarcolemmal ATP-sensitive potassium channels in mouse model
- Inhibition of ATP-Sensitive K^+ Channels and L-Type Ca^ Channels by Amiodarone Elicits Contradictory Effect on Insulin Secretion in MIN6 Cells
- Novel Antiarrhythmic Drugs for the Treatment of Atrial Fibrillation : Current Perspective
- A nomogram predicting disease-free survival in patients with colorectal liver metastases treated with hepatic resection : multicenter data collection as a Project Study for Hepatic Surgery of the Japanese Society of Hepato-Biliary-Pancreatic Surgery
- Hepatic S4a+S5 and bile duct resection for gallbladder carcinoma
- Electropharmacological Profiles of NTC-801, a Novel Selective Acetylcholine-Activated K+ Channel Blocker
- Antiarrhythmic Effects of NTC-801, a Selective Acetylcholine-Activated K+ Channel Blocker, on Experimental Atrial Fibrillation
- The alterations in hepatic microcirculation and Kupffer cell activity after biliary drainage in jaundiced mice
- Pharmacological Treatment of Atrial Fibrillation: An Overview
- Frequency- and voltage-dependent depression of maximum upstroke velocity of action potentials by primenol in guinea pig ventricular muscles.
- Suppression of lysophosphatidylcholine-induced abnormal automaticity by verapamil in canine Purkinje fibers.
- Does Anti-Inflammatory Action of Amiodarone Explain the High Efficacy in Patients With Heart Failure?
- Tonic Block of the Sodium and Calcium Currents by Ketamine in Isolated Guinea Pig Ventricular Myocytes.