Inhibitory effect of cyclosporin A on prolactin synthesis in GH3 cells
スポンサーリンク
概要
- 論文の詳細を見る
Cyclosporin A (CsA), a potent immunosuppressant, is known to have various effects on the endocrine system, including the pituitary gland, the adrenal cortex, the testes, and the pancreatic islets. In this study, the effects of CsA on prolactin (PRL) synthesis and release were investigated in GH3 cells, a clonal strain of rat pituitary tumor. After incubation of confluent GH3 cells with various concentrations of CsA for 24 hr, the PRL content of the media decreased in a dose-dependent manner: by 28.5% with 100 ng/ml CsA (p < 0.01); and 45.8% with 2,000 ng/ml CsA (p < 0.001), compared with control. However, no significant change was observed in the intracellular PRL content. After removal of CsA from the medium, GH3 cells fully recovered normal secretory activity within 24-48 hr, thus indicating that the inhibitory effect of CsA on PRL secretion was reversible. Northern blot analysis revealed a decrease in the PRL mRNA level in cells treated with CsA. In conclusion, these data suggest that CsA inhibits PRL secretion by reducing the rate of biosynthesis. A possible site of action is on PRL gene expression at the level of mRNA transcription.
- 東北大学 : 東北ジャーナル刊行会の論文
- 1996-12-01
著者
-
Takamura Toshinari
金沢大学 大学院医学系研究科恒常性制御学
-
Takamura T
Dep. Of Disease Control And Homeostasis Kanazawa Univ. Graduate School Of Medical Sci. 13-1 Takara-m
-
Takamura Toshinari
Department Of Endocrinology And Metabolism Kanazawa University Graduate School Of Medical Science
-
Takamura Toshinari
金沢大学 第1内科
-
Takamura Toshinari
Dep. Of Disease Control And Homeostasis Kanazawa Univ. Graduate School Of Medical Sci. 13-1 Takara-m
-
Ohta M
Department Of Pediatrics Faculty Of Medicine University Of Yamanashi
-
KOBAYASHI Ken-ichi
the First Department of Internal Medicine, Faculty of Medicine, kanazawa University
-
Yamamoto Yasuhiko
The First Department Of Internal Medicine School Of Medicine Kanazawa University
-
NAGAI YUKIHIRO
The First Department of Internal Medicine, School of Medicine, Kanazawa University
-
OHSAWA KENSOU
The First Department of Internal Medicine, School of Medicine, Kanazawa University
-
OHTA MASAYOSHI
The First Department of Internal Medicine, School of Medicine, Kanazawa University
-
HISADA AZUSA
The First Department of Internal Medicine, School of Medicine, Kanazawa University
-
YAMASHITA HARUHISA
The First Department of Internal Medicine, School of Medicine, Kanazawa University
-
YOSHIZAWA MIYAKO
The First Department of Internal Medicine, School of Medicine, Kanazawa University
-
TAKAMURA TOSHINARI
The First Department of Internal Medicine, School of Medicine, Kanazawa University
-
YAMASHITA Haruhisa
First Department of Internal Medicine, School of Medicine, Kanazawa University
-
Hisada Azusa
The First Department Of Internal Medicine School Of Medicine Kanazawa University
-
Nagai Y
First Department Of Internal Medicine School Of Medicine Kanazawa University
-
Nagai Yukihiro
The First Department Of Internal Medicine School Of Medicine Kanazawa University
-
Ohta Masayoshi
First Department Of Internal Medicine School Of Medicine Kanazawa University
-
Yoshizawa Miyako
The First Department Of Internal Medicine School Of Medicine Kanazawa University
-
Takamura Toshinari
First Department Of Internal Medicine School Of Medicine Kanazawa University
-
Yamashita Haruhisa
First Department Of Internal Medicine School Of Medicine Kanazawa University
-
Ohsawa Kensou
First Department Of Internal Medicine School Of Medicine Kanazawa University
-
Kobayashi Ken-ichi
The Department Of Endocrinology And Metabolism (first Department Of Internal Medicine) Kanazawa Univ
-
Kobayashi Ken-ichi
The First Department Of Internal Medicine Faculty Of Medicine Kanazawa University
-
Kobayashi Kenichi
The First Department Of Internal Medicine Kanazawa University
関連論文
- Transient anti-GAD antibody positivity and acute pancreatitis with pancreas tail swelling in a patient with susceptible haplotype for type 1 diabetes mellitus
- -1138-PLASMA INSULIN LEVELS IN PATIENTS WITH CORONARY ARTERY DISEASE : COMPARISON BETWEEN VASOSPASTIC ANGINA AND EFFORT ANGINA : THE 54th ANNUAL SCIENTIFIC MEETING OF THE JAPANESE CIRCULATION SOCIETY
- Gender Differences in the Association between Anthropometric Indices of Obesity and Blood Pressure in Japanese
- Liver steatosis, but not fibrosis, is associated with insulin resistance in nonalcoholic fatty liver disease
- Selective estrogen receptor modulator raloxifene-associated aggravation of nonalcoholic steatohepatitis.
- Transformation of p53-positive papillary thyroid carcinoma to anaplastic carcinoma of the liver following postoperative radioactive iodine-131 therapy
- -1289-CHOLINERGIC NERVE FUNCTION IN PATIENTS WITH HEART FAILURE, HYPERTENSION, AND DIABETES MELLITUS : THE 54th ANNUAL SCIENTIFIC MEETING OF THE JAPANESE CIRCULATION SOCIETY
- -0387-EFFECTS OF ATROPINE ON REFLEX VASOCONSTRICTION AND VASODILATION IN HUMANS
- -0244-EFFECTS OF ATROPINE, NITROGLYCERIN AND ATRIAL NATRIURETIC PEPTIDE ON CARDIOPULMONARY BAROREFLEXES
- Low-Dose Atropine Attenuates Muscle Sympathetic Nerve Activity in Healthy Humans
- Inhibitory effect of cyclosporin A on prolactin synthesis in GH3 cells
- -0454 EFFECTS OF INSULIN ON ENDOTHELIUM-DEPENDENT VASCULAR RESPONSIVENESS(PROCEEDINGS OF THE 59th ANNUAL SCIENTIFIC MEETING OF THE JAPANESE CIRCULATION SOCIETY)
- Adrenocoritcotropin-independent unilateral adrenocortical hyperplasia with Cushing's syndrome : Immunohistochemical studies of steroidogenic enzymes, ultrastructural examination and a review of the literature
- Subacute Thyroiditis Developed in Identical Twins Two Years Apart
- Serum levels of KL-6 reflect disease activity of interstitial pneumonia associated with ANCA-related vasculitis
- Apheresis Therapy for Prolonged Red Cell Aplasia after Major ABO-Mismatched Bone Marrow Transplantation
- Steroid Pulse Therapy in Lupus Cystitis
- Relationship between Plasma hANP Level and Pretibial Edema by Pioglitazone Treatment
- The transient appearance of anti-GAD antibody in a type 2 diabetic patient with empyema
- Fulminating Onset Type 1 Diabetes with Positivity for Anti-GAD Antibody and Elevated Pancreatic : Exocrine Enzyme Concentrations
- The hepatic circadian clock is preserved in a lipid-induced mouse model of non-alcoholic steatohepatitis
- P648 Predominance of Arterial Baroreflex Over Cardiopulmonary Baroreflex During Ventricular Tachycardia
- -0393-ACTIVATION PATTERNS OF VENTRICULAR TACHYCARDIA LEADING TO VENTRICULAR FIBRILLATION IN CANINE MYOCARDIAL INFARCTION MODEL
- -0390-ANALYSIS ON THE INITIAL ACTIVATION SITES AND ACTIVATION PATTERN OF REPERFUSION ARRHYTHMIAS IN EXPERIMENTAL DOGS
- Two Cases of Transiently TSBAb-Positive Hypothyroidism Induced by Interferon-Alpha Therapy for Chronic Hepatitis C
- 210 SEVERE GROWTH DECELERATION AFTER WITHDRAWAL OF HIGH DOSE GROWTH HORMONE THERAPY IN A BOY WITH IDIOPATHIC SHORT STATURE
- Adenomatous Goiter with Recurrent Thyrotoxicosis
- Immediate-Type Allergy against Human Insulin Associated with Marked Eosinophilia in Type 2 Diabetic Patient
- Severe Hypercholesterolemia in a Double Heterozygote for Lipoprotein Lipase Deficiency (LPL_) and Apolipoprotein ε_4: A Report of a Family with LPL_
- Adrenal Insrfficiency due to Metastatic Hepatocellular Carcinoma
- A Patient with GH-producing Pituitary Adenoma Presenting with a Binasal Superior Quadrantanopsia
- Amyloid Goiter Presented as a Subacute Thyroiditis-Like Symptom in a Patient with Hypersensitivity Vasculitis
- An Adult Case of Neurohypophyseal Ectopy Presenting ACTH Deficiency and Partial GH Deficiency
- A Novel Spermatogenesis Related Factor-2 (SRF-2) Gene Expression Affected by TCDD Treatment
- Effects of Growth Hormone on Testicular Dysfunction Induced by Cyclophosphamide (CP) in GH-Deficient Rats
- Growth Hormone Advances Spermatogenesis in Premature Rats Treated with Gonadotropin-Releasing Hormone Agonist
- Restoration of Seminiferous Tubular Function after Discontinuation of Long-Term Gonadotropin-Releasing Hormone Agonist Administration in Premature Male Rats
- Effects of Growth Hormone and Isulin-Like Growth Factor I on Testosterone Secretion in Premature Male Rats
- Methylprednisolone Pulse Therapy in Two Clinical Types of Crescentic Glomerulonephritis
- The transient appearance of anti-GAD antibody in a type 2 diabetic patient with empyema
- Physical and Pharmacological Regulation of Atrial Natriuretic Peptide Release from the Isolated, Blood-Perfused Right Atrium of the Dog
- Expression of Rat Sperm Flagellum-Movement Associated Protein Genes under 2,3,7,8-Tetrachlorodibenzo-p-dioxin Treatment
- Expression of the Centrosomal Colon Cancer Autoantigen Gene during Spermatogenesis in the Maturing Rat Testis
- Expression of the Ha-ras Suppressor Family Member 5 Gene in the Maturing Rat Testis
- Simultaneously Found Transient Hypothyroidism due to Hashimoto's Thyroiditis, Autoimmune Hepatitis and Isolated ACTH Deficiency after Cessation of Glucocorticoid Administration
- Effect of Interferon-Beta on Thyroid Function in Patients of Chronic Hepatitis C without Preexisting Autoimmune Thyroid Disease
- Adrenocortical Insufficiency Associated with Long-term High-dose Fosfestrol Therapy for Prostatic Carcinoma
- Ischemic colitis probably induced by refractory constipation after voglibose administration in a patient with total gastrectomy
- Exacerbation of Hypothyroidism Following Tumor Necrosis Factor-.ALPHA. Infusion.