Membrane Stretch-Induced Activation of a TRPM4-Like Nonselective Cation Channel in Cerebral Artery Myocytes
スポンサーリンク
概要
- 論文の詳細を見る
Stretch-activated cation channels (SACs) have been observed in many types of smooth muscle cells. However, the molecular identity and activation mechanisms of SACs remain poorly understood. We report that TRPM4-like cation channels are activated by membrane stretch in rat cerebral artery myocytes (CAMs). Negative pressure (≥20 mmHg, cell-attached mode) activated single channels (approximately 20 pS) in isolated CAMs. These channels were permeable to Na+ and Cs+ and inhibited by Gd3+ (30 μM) and DIDS (100 μM). The effect of negative pressure was abolished by membrane excision, but subsequent application of Ca2+ (>100 nM) to the intracellular side of the membrane restored single channel activity that was indistinguishable from SACs. Caffeine (5 mM), which depletes SR Ca2+-stores, first activated and then abolished SACs. Tetracaine (100 μM), a ryanodine receptor antagonist, inhibited SACs. Overexpression of hTRPM4B in HEK293 cells resulted in the appearance of cation channels that were activated by both negative pressure and Ca2+ and which had very similar biophysical and pharmacological properties as compared with SACs in CAMs. These studies indicate that TRPM4-like channels in CAMs can be activated by membrane stretch, possibly through ryanodine receptor activation, and this may contribute to the depolarization and concomitant vasoconstriction of intact cerebral arteries following mechanical stimulation.
- 社団法人 日本薬理学会の論文
- 2007-04-20
著者
-
Nelson Mark
Department of Pharmacology, College of Medicine, University of Vermont
-
Brayden Joseph
Department of Pharmacology, College of Medicine, University of Vermont
-
Inoue Ryuji
Department of Physiology, School of Medicine, Fukuoka University
-
ITO Yushi
Department of Pharmacology, Graduate School of Medical Sciences, Kyushu University
-
Ito Y
Department Of Pharmacology Graduate School Of Medical Sciences Kyushu University
-
Nelson Mark
Department Of Pharmacology College Of Medicine University Of Vermont
-
MORITA Hiromitsu
Department of Pharmacology, College of Medicine, University of Vermont
-
HONDA Akira
Department of Pharmacology, College of Medicine, University of Vermont
-
ABE Kihachiro
Special Patient Oral Care Unit, Kyushu University Hospital
-
Inoue Ryuji
Department Of Physiology School Of Medicine Fukuoka University
-
Inoue Ryuji
Department Of Pharmacology Faculty Of Medicine Kyushu University
-
Abe Kihachiro
Special Patient Oral Care Unit Kyushu University Hospital
-
Brayden Joseph
Department Of Pharmacology College Of Medicine University Of Vermont
-
Ito Yushi
Department Of Neonatology National Center For Child Health And Development
-
Honda Akira
Department Of Pharmacology College Of Medicine University Of Vermont
-
Honda Akira
Department Of Chest Medicine School Of Medicine Chiba University
-
Inoue Ryuji
Special Patient Oral Care Unit Kyushu University Hospital
-
Morita Hiromitsu
Special Patient Oral Care Unit Kyushu University Hospital
-
Ito Yushi
Department of Health Science, Kumamoto Health Science University
関連論文
- Effects of Z-338, a novel gastroprokinetic agent, on the actions of excitatory and inhibitory neurotransmitters on neurons in area postrema
- ラット大脳動脈における張力活性化型,細胞内Ca^感受性,非特異的陽イオンチャネル(一般演題「イオンチャネル1」)
- DPE-007 Protein Kinase G-mediated Inhibition of TRPC6 Channel Activity Participates in Anti-hypertrophic Effect of Cardiac Natriuretic Peptides(DPE02,Heart Failure, Basic (M),Digital Poster Session (English),The 73rd Annual Scientific Meeting of The Japan
- Membrane Stretch-Induced Activation of a TRPM4-Like Nonselective Cation Channel in Cerebral Artery Myocytes
- Potassium Channels and Neurovascular Coupling
- The associated markers and their limitations for the primary screening of HCV carriers in public health examination
- Regulatory T cells and liver pathology in a murine graft versus host response model
- HPC-1/Syntaxin-1A Activity in the Enteric Nervous System of Developing Rat Gastrointestinal Tract
- Association of vitamin D and estrogen receptor gene polymorphism with the effects of longterm hormone replacement therapy on bone mineral density
- Effect of vitamin D receptor and estrogen receptor gene polymorphism on the relationship between dietary calcium and bone mineral density in Japanese women
- Dual Signaling Pathways of Arterial Constriction by Extracellular Uridine 5′-Triphosphate in the Rat
- Roles of the Dimethylamino Group and Macrodiolide Ring of Pamamycin-607 in Its Aerial Mycelium-inducing Activity
- Discrepancies in cytogenetic results between amniocytes and postnatally obtained blood : trisomy 9 mosaicism
- Gene Expression of a Circadian Pacemaker Hormone Peptide PDF in Cricket Gryllus bimaculatus
- Involvement of TRPM7 in Cell Growth as a Spontaneously Activated Ca^ Entry Pathway in Human Retinoblastoma Cells
- Current Perspective : Dissecting Receptor-Mediated Ca^ Influx Pathways : TRP Channels and Their Native Counterparts
- Transient Receptor Potential Protein as a Novel Non-Voltage-Gated Ca^ Entry Channel Involved in Diverse Pathophysiological Functions
- Accumulation of 7α-hydroxycholesterol in liver tissue of patients with cholesterol gallstones
- Increased bile acid concentration in liver tissue with cholesterol gallstone disease
- A murine model of pulmonary basidiomycosis by Schizophyllum commune
- Inhaled nitric oxide and extracorporeal membrane oxygenation in persistent pulmonary hypertension of the newborn
- Reconstitution in Lipid Bilayer of Smooth Muscle Cation Channels Activated Through a GTP-Binding Protein
- 17β-Estradiol-induced enhancement of estrogen receptor biosynthesis via MAPK pathway in mouse skeletal muscle myoblasts
- Glycolytic ATP Production Regulates Muscarinic Cation Currents in Guinea-pig Ileum
- MODULATION AND IONIC MECHANISMS INVOLVED IN AUTONOMIC NEUROEFFECTOR TRANSMISSION
- Effects of Nitrogen and Titanium on Mechanical Properties and Annealed Structure of Ar-N_2 Gas Metal Arc Welded Copper
- Effects of Nitrogen and Titanium on Porosity and Microstructure in Ar-N_2 Gas Metal Arc Welded Copper
- Simultaneous Activation of Ca^-Dependent K^+ and Cl^- Currents by Various Forms of Stimulation in the Membrane of Smooth Muscle Cells from the Rabbit Basilar Artery
- Comparative Studies on the Relaxing Action of Several Adenosine 5'-Triphosphate-Sensitive K^+ Channel Openers in Pig Urethra
- Fenamates Potentiate the α_1-Adrenoceptor-Activated Nonselective Cation Channels in Rabbit Portal Vein Smooth Muscle
- Possible Involvement of M_5 Muscarinic Receptor in the Enhancing Actions of the Novel Gastroprokinetic Agent Z-338 on Nifedipine-Sensitive Voltage-Dependent Ca^ Currents in Guinea Pig Stomach
- Potentiating Actions of Lanthanum on ACh-Induced Cation Current in Guinea-Pig Ileal Smooth Muscle Cells
- EFFECTS OF PHTHALAZINOL, EG 626, ON MEMBRANE AND MECHANICAL PROPERTIES OF SMOOTH MUSCLE CELLS OF RABBIT MAIN PULMONARY ARTERY AND SUPERIOR MESENTERIC ARTERY
- Dual Signaling Pathways of Arterial Constriction by Extracellular Uridine 5'-Triphosphate in the Rat
- Two Types of K^+ Currents Underlying Inward Rectification of Rat Osteoclast Membrane : A Single-Channel Analysis
- ECHOCARDIOGRAPHIC EVALUATION OF CRITICAL HEART DISEASE IN NEONATE
- POLYCHLORBIPHENYLS (PCB) INDUCED FETOPATHY:I. CLINICAL OBSERVATION
- POLYCHLOROBIPHENYLS (PCB) INDUCED FETOPATHY:II. EXPERIMENTAL STUDIES : POSSIBLE PLACENTAL TRANSFER OF POLYCHLOROBIPHENYLS IN RATS
- Involvement of TRPM7 in Cell Growth as a Spontaneously Activated Ca2+ Entry Pathway in Human Retinoblastoma Cells
- Cholesterol and chronic hepatitis C virus infection
- Hepatitis C virus infection causes hypolipidemia regardless of hepatic damage or nutritional state : An epidemiological survey of a large Japanese cohort