Combination of Mixed Self-Assembled Monolayer and Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry, a Simple Tip-Based Screening Method for Proteomics
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概要
- 論文の詳細を見る
A new screening method to detect protein-ligand interactions has been developed through a combination of mixed self-assembled monolayer (SAM) and matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS). The mixed SAM surface was prepared on flat gold plate from a mixture of 20-mercapto-3,6,9-trioxaicosanol and a ligand-bound derivative of 29-mercapto-3,6,9,12,15,18-hexaoxanonacosanol. This surface is very suitable for observing the molecular interaction accurately, because there is very low levels of steric hindrance between immobilized ligands and the non-charged hydroxyl groups of polyethylene glycol which resists from the non-specific adsorption of biomolecules. Because the surface coated with the mixed SAM is washable with water, it can be used directly as a probe for MALDI-MS, a technique commonly used for the annotation of protein function. As an example we report here the selective detection of the molecular interaction between a Src homology 3 (SH3) domain of human p85α phosphatidylinositol 3-kinase (PI3K) and its ligand peptide (H-RKLPPRPAF-OH) attached covalently to mixed SAM using MALDI-MS.
- 日本質量分析学会の論文
- 2006-10-01
著者
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Hirota Hiroshi
RIKEN Genomic Sciences Center
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Hirota Hiroshi
Protein Research Group Riken Genomic Sciences Center And Division Of Supramolecular Biology Yokohama
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Asanuma Miwako
Protein Research Group Riken Genomic Sciences Center
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TACHIBANA Kazuo
Department of Chemistry, School of Science, The University of Tokyo
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Fukuzawa Seketsu
Department Of Chemistry School Of Science The University Of Tokyo And Crest Japan Science And Techno
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FUKUZAWA Seketsu
Protein Research Group, RIKEN Genomic Sciences Center
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Tachibana Kazuo
Department Of Chemistry School Of Science The University Of Tokyo And Crest Japan Science And Techno
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Tachibana Kazuo
Department Of Chemistry School Of Science The University Of Tokyo
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Hirota Hiroshi
Protein Research Group Riken Genomic Science Center
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