Differential Effects of Buckwheat and Kudingcha Extract on Neuronal Damage in Cultured Hippocampal Neurons and Spatial Memory Impairment Induced by Scopolamine in an Eight-Arm Radial Maze

概要

論文の詳細を見る
We have reported the neuroprotection provided by an extract of buckwheat (BWE, Fagopyrum esculentum Moench) on neuronal damage in the repeated cerebral ischemia model and demonstrated that BWE inhibited the excess glutamate release induced by repeated cerebral ischemia, suggesting that BWE had a free radical-scavenging in addition to anti-glutamate action. In the present study, we studied the neuroprotective effects of Kudingcha extract (KDE, Ligustrum purpurascens Y.C.) on scavenging by the 2, 2-diphenyl-1-picylhydrazyl (DPPH) radical in primary cultured hippocampal neurons, and on spatial memory impairment induced by scopolamine in an eight-arm radial maze in comparison with BWE. The effects of KDE (0.01-1mg/ml) were more potent than those of BWE (0.01-1mg/ml) in scavenging the DPPH radical (1mM). KDE (100μg/ml) prevented the cell damage induced by glutamate (300μM) or kainate (1mM), which was more potent than BWE (100μg/ml), but BWE suppressed the cellular damage induced by β-amyloid_<(25-35)> (20μM) more potently than KDE (100μg/ml). BWE (600mg/kg), but not KDE, significantly suppressed the increase in errors induced by scopolamine (0.5mg/kg i.p.) in the eight-arm radial maze. The results suggest that BWE may protect against cholinergic dysfunction and that KDE protects more effectively against glutaminergic dysfunction.
社団法人日本薬学会の論文
2005-12-01