Synthesis of DNA Conjugate by Mechanochemical Solid-State Polymerization and Its Affinity Separation of Oligonucleotides Having Single-Base Difference by Capillary Electrophoresis

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In this communication, we discuss the characterization of DNA conjugate synthesized by mechanochemical polymerization, Con-M, on the separation of model oligo-DNA and its single nucleotide polymorphisms (SNPs) by affinity capillary electrophoresis, compared with that prepared by radical-initiated solution polymerization, Con-RL. The average molecular weight of Con-M was similar to that of Con-RL, although the molecular weight distribution of Con-M was narrower than that of Con-RL. Capillary electrophoresis of oligo-DNA was performed using the capillary filled with DNA conjugate. The resolution of the capillary filled with Con-M was apparently higher than with Con-RL. It is considered that higher resolution using the capillary filled with Con-M could be ascribed not only to the narrow molecular weight distribution but also to the difference of copolymer structure.
公益社団法人日本薬学会の論文
2005-07-01