Metabolic Fingerprinting of Ephedra Species Using ^1H-NMR Spectroscopy and Principal Component Analysis

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概要

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• The metabolomic analysis of Ephedra species was performed using ^1H-NMR spectroscopy and multivariate data analysis. A broad range of metabolites could be detected by ^1H-NMR spectroscopy without any chromatographic separation. The principal component analysis used to reduce the huge data set obtained from the ^1H-NMR spectra of the plant extracts clearly discriminated three different Ephedra species. The major differences in Ephedra sinica, Ephedra intermedia and Ephedra distachya var. distachya were found to be due to benzoic acid analogues in the aqueous fraction and ephedrine-type alkaloids in the organic fraction. Based on this metabolomic recognition, one of nine commercial Ephedra materials evaluated was shown to be a mixture of Ephedra species. This method will be a useful tool for chemotaxonomic analysis and authentification of Ephedra species including quality control of plant materials.

• 公益社団法人日本薬学会の論文
• 2005-01-01

著者

• Choi Young

  Division of Gynecologic Oncology, Department of Obstetrics and Gynecology, Yonsei University College

• Choi Y

  Leiden Univ. Leiden Nld

• Erkelens Cornelis

  Amsterdam Center For Drug Research Gorlaeus Laboratories Leiden University

• Kim Hye

  Division Of Pharmacognosy Institute Of Biology Leiden University

• Choi Young

  Division Of Functional Genomics Jichi Medical University

• VERPOORTE Robert

  Division of Pharmacognosy, Leiden/Amsterdam Center for Drug Research, Gorlaeus Laboratories

• Verpoorte R

  Leiden Univ. Leiden Nld

• Verpoorte Robert

  Division Of Pharmacognosy Center For Bio-pharmaceutical Sciences Leiden University

• ERKELENS Cornelis

  Division of NMR, Leiden Institute of Chemistry, Gorlaeus Laboratories, Leiden University

• LEFEBER Alfons

  Division of NMR, Leiden Institute of Chemistry, Gorlaeus Laboratories, Leiden University

• Lefeber Alfons

  Division Of Nmr Leiden Institute Of Chemistry Gorlaeus Laboratories Leiden University

• Kim Hye

  Division Of Nephrology Department Of Internal Medicine Korea University Anam Hospital Korea Universi

• KIM Hye

  Division of Endocrinology and Metabolism, Department of Medicine, Samsung Medical Center, Sungkyunkwan University School of Medicine

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