Concordance between results of serum protein electrophoresis of pathological serum by cellulose acetate and capillary zone electrophoresis

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概要

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• We investigated whether the electrophoretic patterns and clinical information derived from the five principal fractions of the Beckman Paragon capillary zone electrophoresis (CZE) 2000 system are comparable to the results obtained by the cellulose acetate electrophoresis (CAE: Olympus AES 320) system. Concordance studies between the results obtained by CAE and CZE were performed on sera from pregnant women and from patients with nephrotic syndrome, acute and chronic inflammatory diseases, cirrhosis, monoclonal and polyclonal gammopathy, and bisalbuminemia. In all subjects except for the pregnant women, the results of CAE and CZE agreed 100% in terms of percent composition for albumin, and α1- and α2-globulins. Concordance for γ-globulin was 92-100% in terms of both percent composition and absolute concentration. Three discordant cases according to the results for β-globulin by CAE and CZE were found in patients with monoclonal gammopathy, who also showed discordant results for γ-globulin. An M-protein spike observed in either the β-globulin or γ-globulin fraction was 100% detected by both CAE and CZE analyses. In another six discordant cases for the β-globulin fraction observed in patients with cirrhosis and polyclonal gammopathy, part of the tail of the β-globulin fraction tended to be measured as γ-globulin by CAE. In three of the four bisalbuminemia cases, the double band of albumin observed by CZE was not detected by CAE. The results show that the electrophoretic patterns and clinical information derived from the five principal fractions of the Beckman Paragon CZE 2000 system are comparable to the results obtained by classical CAE.

• 2005-06-01

著者

• 渭原 博

  東邦大学医療センター大橋病院臨床検査部

• Yoshida M

  Toho Univ.

• IHARA Hiroshi

  Departments of Laboratory Medicine, Ohashi and Omori Hospitals, School of Medicine, Toho University

• MATSUMOTO Takayuki

  Departments of Laboratory Medicine, Ohashi and Omori Hospitals, School of Medicine, Toho University

• KAKINOKI Takashi

  Departments of Laboratory Medicine, Ohashi and Omori Hospitals, School of Medicine, Toho University

• AOKI Yutaka

  Departments of Laboratory Medicine, Ohashi and Omori Hospitals, School of Medicine, Toho University

• HASHIZUME Naotaka

  Departments of Laboratory Medicine, Ohashi and Omori Hospitals, School of Medicine, Toho University

• INADA Yoshinobu

  Departments of Laboratory Medicine, Ohashi and Omori Hospitals, School of Medicine, Toho University

• NANBA Shunji

  Departments of Laboratory Medicine, Ohashi and Omori Hospitals, School of Medicine, Toho University

• YOSHINO Gen

  Departments of Laboratory Medicine, Ohashi and Omori Hospitals, School of Medicine, Toho University

• YOSHIDA Mitsutaka

  Department of Environmental and Biotechnological Frontier Engineering, Fukui University of Technolog

• Aoki Yutaka

  Department Of Laboratory Medicine Ohashi Hospital Toho University School Of Medicine

• Nanba Shunji

  Departments Of Laboratory Medicine Ohashi And Omori Hospitals School Of Medicine Toho University

• Ihara H

  Department Of Laboratory Medicine Ohashi Hospital School Of Medicine

• Ihara Hiroshi

  Department Of Laboratory Medicine Ohashi Hospital Toho University School Of Medicine

• Ihara Hiroshi

  Departments Of Laboratory Medicine Ohashi And Omori Hospitals School Of Medicine Toho University

• Yoshino Gen

  Department Of Diabetes And Endocrinology Toho University Omori Medical Center

• Inada Yoshinobu

  Departments Of Laboratory Medicine Ohashi And Omori Hospitals School Of Medicine Toho University

• Yoshida Mitsutaka

  Department Of Biology Faculty Of Science Toho University

• Yoshida Mitsutaka

  Department Of Biological Science Aichi University Of Education

• Hashizume Naotaka

  Department Of Laboratory Medicine Ohashi Hospital School Of Medicine

• Hashizume Naotaka

  Department Of Laboratory Medicine Ohashi Hospital Toho University School Of Medicine

• Hashizume Naotaka

  東邦大学医学部附属大橋病院検査部

• Hashizume Naotaka

  Department Of Clinical Pathology School Of Medicine Teikyo University

• Kakinoki Takashi

  Department Of Laboratory Medicine Ohashi Hospital Toho University School Of Medicine

• Matsumoto Takayuki

  Department Of Gastroenterology Osaka City University Graduate Medical School

• Yoshino Gen

  Departments Of Laboratory Medicine Ohashi And Omori Hospitals School Of Medicine Toho University

• Matsumoto Takayuki

  Department Of Gastroenterology And Hepatology Institute Of Clinical Medicine Graduate School Of Comp

• Matsumoto Takayuki

  Department Of Electronics Information And Communication Engineering Waseda University

• Matsumoto Takayuki

  Departments Of Laboratory Medicine Ohashi And Omori Hospitals School Of Medicine Toho University

• IHARA Hiroshi

  Department of Laboratory Medicine Ohashi Hospital

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