グルカナーゼ遺伝子またはキチナーゼ遺伝子を導入した組換えキウイフルーツがアレロパシー物質の生産性および土壌微生物相に及ぼす影響(育種・遺伝資源)

概要

論文の詳細を見る
The influence of transformed kiwifruit with glucanase or chitinase genes on the non-rhizosphere and rhizosphere soil were investigated to accumulate basic information in terms of allelopathic effects and soil microflora. When the allelopathic effects were evaluated using lettuce sprouts that are sensitive to allelopathic substances, there was no detectable difference in the growth of the lettuce sprouts with root and shoot tissue of both transformants and non-transformants. There was also no difference on the soil microflora of pot soil between transformants and non-transformants, in which the number of mold fungi, actinomycetes and bacteria in the non-rhizosphere and rhizosphere were not significantly different from transformants and non-transformants. Kanamycin-resistant bacteria were present in soils from both transformants and non-transformants. To identify the origin of kanamycin-resistance, PCR using primer combinations corresponding to transformant-specific marker genes were carried out. The results showed that the kanamycin-resistant bacteria were not due to horizontal gene transfer of transgenes from transformants to microorganism, indicating that these bacteria were present in the soil regardless of transformants or non-transformants. Collectively, these results suggested that the influences of transformed kiwifruit with glucanase or chitinase genes on the non- rhizosphere and rhizosphere soil are substantially the same as those of non-transformants.
園芸学会の論文
2004-12-15

グルカナーゼ遺伝子またはキチナーゼ遺伝子を導入した組換えキウイフルーツがアレロパシー物質の生産性および土壌微生物相に及ぼす影響(育種・遺伝資源)

スポンサーリンク

概要

著者

関連論文

スポンサーリンク