活性汚泥からの ANAMMOX 微生物の集積培養

概要

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Anaerobic ammonium removal was observed in batch tests using a denitrifying sludge from an industrial wastewater treatment plant and an intermittent nitrifying-denitrifying sludge from a mixed piggery domestic wastewater treatment plant after 92 and 84 days, respectively. The simultaneous removals of ammonium and nitrite with nitrate production at ratios corresponding to that reported for the Anammox process were observed. Their specific ammonium conversion rates following one year of incubation after the first detection were 0.260 and 0.281 g N·g protein-d-1, respectively. Using 16S rDNA sequence analysis, one of the sludges was confirmed to have an organism with a 99.2% similarity to the first reported Anammox bacterium, a deeply branching planctomycete, Candidatus Brocadia anammoxidans. In addition, the other sludge contained an organism with a 98.9% similarity to the Anammox planctomycete, Candidatus Kuenenia stuttgartiensis.
2004-06-10