Genetic Typing of the Mouse and Rat Nude Mutations by PCR and Restriction Enzyme Analysis
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概要
- 論文の詳細を見る
A genetic typing method for the mouse and rat nude mutations by PCR and restriction fragment length polymorphism (RFLP) analysis was developed. Since restriction sites useful for RFLP analysis do not exist in the mouse nu and rat rnu mutations, artificial restriction sites were introduced by PCR with modified primers. Three genotypes in the mouse (nu/nu, nu/+ and +/+) or rat (rnu/rnu, rnu/+ and +/+) are rapidly differentiated with the PCR-RFLP assay. In addition, congenic nude strains can be efficiently established by using this assay. Finally, genetic mapping of the rnu locus was performed with microsatellite markers. The locus order on rat chromosome 10 was D10Mgh14-(2.0cM)-D10Mit2-(1.4cM)-rnu-(0.7cM)-D10Mgh6-(2.7cM)-D10Mit8.
- 社団法人 日本実験動物学会の論文
著者
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Makino Susumu
Aburahi Laboratories Shionogi & Co. Ltd.
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Makino S
Shionogi And Co. Ltd. Shiga Jpn
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Makino S
Shionogi Research Laboratories Shionogi & Co. Ltd.
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Makino Susumu
Shionogi Research Laboratories Shionogi & Co. Ltd.
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Makino Susumu
Center For Experimental Animals Development Shionogi & Co. Ltd.
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HIRASAWA Tsutomu
Center for Experimental Animals Development, Shionogi & Co., Ltd.
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YAMASHITA Hirofumi
Center for Experimental Animals Development, Shionogi & Co., Ltd.
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Yamashita Hirofumi
Shionogi Research Laboratories Shionogi & Co. Ltd.
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Hirasawa T
Shionogi Research Laboratories Shionogi & Co. Ltd.
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Hirasawa Tsutomu
Center For Experimental Animals Development Shionogi & Co. Ltd.
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Yamashita H
Shionogi Research Laboratories Shionogi & Co. Ltd.
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Horikawa Tatsuya
Department Of Dermatology Kobe University School Of Medicine
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