Genetic Typing of the Mouse ob Mutation by PCR and Restriction Enzyme Analysis

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概要

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• A genetic typing method for the mouse obese (ob) mutation by PCR and restriction fragment length polymorphism (RFLP) analysis was developed. Three genotypes (ob/ob, ob/+ and +/+) of the ob mouse are rapidly differentiated with this assay. Since only a small biopsy specimen (tail end) is necessary for the genotyping, the ob mouse at any age is typable and can be used in subsequent breeding or research. Obese homozygotes (ob/ob) were efficiently produced by mating heterozygotes (ob/+) only, which were selected by using the PCR-RFLP assay.

• 社団法人　日本実験動物学会の論文

著者

• Makino Susumu

  Center For Experimental Animals Development Shionogi & Co. Ltd.

• HIRASAWA Tsutomu

  Center for Experimental Animals Development, Shionogi & Co., Ltd.

• OHARA Tadao

  Center for Experimental Animals Development, Shionogi & Co., Ltd.

• Ohara Tadao

  Center For Experimental Animals Development Shionogi & Co. Ltd.

• Hirasawa Tsutomu

  Center For Experimental Animals Development Shionogi & Co. Ltd.

関連論文

• Genetic Typing of the Mouse ob Mutation by PCR and Restriction Enzyme Analysis
• Genetic Typing of the Mouse and Rat Nude Mutations by PCR and Restriction Enzyme Analysis

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