Suitability of AFLP markers for the study of Genetic relationships among Korean native dogs
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概要
- 論文の詳細を見る
To determine the genetic relationships among domestic dog breeds, we performed both a sequence comparison of mitochondrial DNA (mtDNA) and an amplified fragment length polymorphisms (AFLP) analysis. Three of four regions of mtDNA, cytochrome b, cytochrome oxidase subunit II, and 16S rRNA genes were highly homogeneous among dog breeds, whereas the other region, the control region, showed relatively high polymorphisms with a maximum percentage difference of 3.18%. However, the control region showed extensive polymorphism even within breeds, and the relationship tree derived from the data could not clearly delimit distinct breeds. 19 EcoRI/MseI primer combinations were used to generate AFLP markers among 25 dogs from 11 breeds including three Korean native dogs. These amplification reactions allowed the detection of more than 1900 amplification products of which 408 were identified as polymorphic bands. Unrooted neighbor-joining tree based on dissimilarity values showed that the Korean native dogs were clustered together with the Asian dogs and that the Asian originated dogs were clustered separately from Western originated dogs. A consensus tree using parsimony method also showed Korean native dogs were grouped separately from the other dogs with moderate bootstrap values. Taken together, it is concluded that AFLP analysis is a more informative tool for revealing genetic relationships among dog breeds than mtDNA sequence comparsion.
- 日本遺伝学会の論文
著者
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Hong Ha
Department Of Genetic Engineering Kyungpook National University
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SEOK KIM
Department of Genetic Engineering, Kyungpook National University
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WON JEONG
Department of Genetic Engineering, Kyungpook National University
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KYU PARK
National Creative Research Initiative Center for Behavioral Genetics, Department of Biological Scien
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Kim Kyung
Department Of Anesthesia And Pain Medicine School Of Medicine Pusan National University
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Won Jeong
Department Of Genetic Engineering Kyungpook National University
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Seok Kim
Department Of Genetic Engineering Kyungpook National University
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Park Chan
National Genome Research Institute Nih
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Kyu Park
National Creative Research Initiative Center For Behavioral Genetics Department Of Biological Scienc
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Jeong Ho
Department of Genetic Engineering, Kyungpook National University
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Jeong Ho
Department of Advanced Materials Engineering, Sungkyunkwan University, Suwon, Gyeonggi 440-746, Republic Korea
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