CATHODIC ELECTROCHEMILUMINESCENCE OF LUMINOL ENHANCED BY ANTIBODY-ANTIGEN REACTION
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概要
- 論文の詳細を見る
The purpose of the present work is to characterize the cathodic electrochemiluminescence of luminol with a view to its application in continuous immunoassay. The authors designed a method for continuous antibody determination from the change in electrochemilumineseent intensity of luminol resulted from antigen-antibody reactions. The cathodic clectrochemiluminescences of lurninol-labeled antigens in hydrogen peroxide solution were enhanced by the presence of the respective antibodies. Cathodic clectruchemiluminescence of a luminol-labeled antibody was also enhanced by the presence of antigen. The enhancement of electrochemiluminescence may be useful in homogeneous inummoassay of antibodies or antigens. Analysis of a batchwise reaction suggested that the presenee of antibody increases the quantum yield of electrochemiluminescence of luminol-labeled antigen. And analysis of the electrolytic current of luminol and hydrogen peroxide at indium-tin oxide (ITO) cathode revealed that hydrogen peroxide was electrochemically active at the cathode, while lummol was inert. These results suggest that cathodic electrochemiluminescence of luminol or luminol-labeled proteins is triggered by hydroxide radical produced from hydrogen peroxide at the ITO cathode.
- 社団法人 化学工学会の論文
- 1996-10-20
著者
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Sakai Kiyotaka
Department Of Chemical Engineering Waseda University
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Sakai K
Department Of Chemical Engineering Waseda University
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Miyasaka Takehiro
Department Of Medical Engineering Himeji Dokkyo University
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Miyasaka Takehiro
Department Of Cardiovascular Physiology Olayama Graduate School Of Medicine Dentistry And Pharmaceut
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YOSHIMI Yasuo
Department of Industrial Chemistry, Shibaura Institute of Technology
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Miyasaka Takehiro
Department Of Chemical Engineering Waseda University
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Yoshimi Yasuo
Department Of Industrial Chemistry Shibaura Institute Of Technology
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Yoshimi Yasuo
Department Of Applied Chemistry Shibaura Institute Of Technology
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HARAMOTO Hirotaka
Department of Chemical Engineering, Waseda University
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Haramoto Hirotaka
Department Of Chemical Engineering Waseda University
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Miyasaka Takehiro
Department Of Cardiovascular Physiology Okayama University Graduate School Of Medicine And Dentistry
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SAKAI KIYOTAKA
Department of Applied Chemistry, Faculty of Science and Engineering, Waseda University
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