Application of Organosilane Monolayer Template to Quantitative Evaluation of Cancer Cell Adhesive Ability
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概要
- 論文の詳細を見る
The adhesive ability of two human pancreatic cancer cell lines was evaluated using organosilane monolayer templates (OMTs). Using the OMT, the spreading area of adhered cells can be limited, and this enables us to focus on the initial attachment process of adhesion. Moreover, it becomes possible to arrange the cells in an array and to quantitatively evaluate the number of attached cells. The adhesive ability of the cancer cells cultured on the OMT was controlled by adding (-)-epigallocatechin-3-gallate (EGCG), which blocks a receptor that mediates cell adhesion and is overexpressed in cancer cells. Measurement of the relative ability of the cancer cells to attach to the OMT revealed that the ability for attachment decreased with increasing EGCG concentration. The results agreed well with the western blot analysis, indicating that the OMT can potentially be employed to evaluate the adhesive ability of various cancer cells.
- 2011-06-25
著者
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Tanii Takashi
School Of Science And Engineering Waseda University
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Sasaki Kosuke
School of Science and Engineering, Waseda University, Shinjuku, Tokyo 169-8555, Japan
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Ichisawa Kota
School of Science and Engineering, Waseda University, Shinjuku, Tokyo 169-8555, Japan
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Demura Takanori
School of Science and Engineering, Waseda University, Shinjuku, Tokyo 169-8555, Japan
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Beppu Yuichi
School of Science and Engineering, Waseda University, Shinjuku, Tokyo 169-8555, Japan
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Vu Hoan
Research Institute of National Center for Global Health and Medicine, Shinjuku, Tokyo 162-8602, Japan
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Chi Hoan
Research Institute of National Center for Global Health and Medicine, Shinjuku, Tokyo 162-8602, Japan
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Yamamoto Hideaki
School of Science and Engineering, Waseda University, Shinjuku, Tokyo 169-8555, Japan
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Sato Yuko
Research Institute of National Center for Global Health and Medicine, Shinjuku, Tokyo 162-8602, Japan
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