Characterization of Dipalmitoylphosphatidylcholine/Cholesterol Langmuir-Blodgett Monolayers Investigated by Atomic Force Microscopy and Fourier Transform Infrared Spectroscopy
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概要
- 論文の詳細を見る
The addition effects of cholesterol on the dipalmitoylphosphatidylcholine (DPPC) Langmuir-Blodgett (LB) monolayer have been investigated by atomic force microscopy (AFM) and infrared reflection absorption spectroscopy (IRRAS). The phase transformation from pure DPPC to the DPPC/cholesterol phase proceeds through two stages: initial drastic changes in the surface morphology and the conformation of the DPPC acyl chains below 10% cholesterol, and the gradual homogenization of the morphology towards the liquid-order phase up to 35% cholesterol. The IRRAS peak position indicates that the conformational disorder of the acyl chain becomes almost that of the liquid level at 10% cholesterol addition. In the homogeneous liquid-order phase at 35% cholesterol, the terminal methyl groups of the DPPC are aligned in good order similarly to the solidlike gel phase, whereas the acyl chains have a liquid-level disordered conformation.
- Published by the Japan Society of Applied Physics through the Institute of Pure and Applied Physicsの論文
- 2004-06-15
著者
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Kim Yong-hoon
The Graduate University For Advanced Studies Institute For Molecular Science
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TERO Ryugo
Institute for Molecular Science
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URISU Tsuneo
The Graduate University for Advanced Studies, Institute for Molecular Science
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Takizawa Morio
The Graduate University For Advanced Studies Institute For Molecular Science
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Kim Yong-Hoon
The Graduate University for Advanced Studies, Institute for Molecular Science, Myodaiji, Okazaki 444-8585, Japan
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Tero Ryugo
Institute for Molecular Science, Myodaiji, Okazaki 444-8585, Japan
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Urisu Tsuneo
The Graduate University for Advanced Studies, Institute for Molecular Science, Myodaiji, Okazaki 444-8585, Japan
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