Interaction and Orientation of an .ALPHA.-Aminoisobutyric Acid- and Tryptophan-Containing Short Helical Peptide Pore-Former in Phospholipid Vesicles, as Revealed by Fluorescence Spectroscopy.
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概要
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The interaction and orientation of a membrane protein ion channel model, an α-aminoiso-butyric acid analogue of gramicidin B (GBA), in egg yolk phosphatidylcholine vesicles was studied by means of fluorescence spectroscopic techniques. GSA helices form stable ionconducting pores in membranes [Jelokhani-Niaraki et al. (1995) J. Chem. Soc. Perkin Trans. 2, 801-808]. In an α-helical model for the peptide, all Trp residues (intrinsic fluorophores) are distributed near the C-terminus. Fluorescence quenching experiments revealed the exposure of the helical peptides' C-termini to aqueous environments. Dansyllabeled vesicles were used to investigate the GBA dynamism of the interaction with membranes. It was shown that considerable amounts of peptide reside on and in the vicinity of the outer surface of lipid bilayers. The transmembrane transfer to the inner layer is slow due to the high affinity of Trp residues for bilayer interfaces which anchor the peptide to the outer surface. A structural-functional interpretation of the GBA interaction with membranes is presented.
- 社団法人 日本生化学会の論文
著者
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Nakashima Kenichi
Department Of Chemistry Faculty Of Science And Engineering Saga University
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KONDO Michio
Department of Chemistry, Faculty of Science and Engineering, Saga University
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Kodama Hiroaki
Department Of Anatomy Ohu University School Of Dentistry
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Kondo Michio
Department Of Chemistry Faculty Of Science And Ehgineering Saga University
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Jelokhani-Niaraki Masood
Department of Chemistry, Faculty of Science and Engineering, Saga University
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