Chemical Modification of Subtilisin BPN' by 5-Diazo-1-H-Tetrazole
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概要
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1. Subtilisin BPN' [EC 14. 4. 16], a bacterial protease, was chemically modified with 6-diazo-l-H-tetrazole (DHT) and the nature of the modified enzyme was investigated. 2. Subtilisin modified with 19 to 60 fold molar excesses of DHT remarkably decreased its hydrolytic activity toward N-acetyl-tyrosine ethyl ester, but not so notably the activity toward casein. This effect was correlated to the modification of tyrosine residue of the enzyme. 3. Modification of one critical histidine residue by DHT brought about the inactivation of the enzymatic activities, and this inactivated enzyme failed to incorporate an irreversible inhibitor, diisopropyl phosphorofluoridate.
- 社団法人 日本生化学会の論文
著者
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Ohtsuki Kozo
Department Of Food Science And Nutrition Kyoto Prefectural University
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Hatano Hiroyuki
Department of Chemistry, Faculty of Science, Kyoto University
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KAJIWARA Nobuko
Department of Chemistry, Faculty of Science, Kyoto University
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