Dynamic structure of pyrimidine-polyribonucleotides in solution. 1HNMR studies.
スポンサーリンク
概要
- 論文の詳細を見る
Temperature dependent conformations and molecular motions of pyrimidine polyribonucleotides, poly(C) and poly(U), have been studied in <SUP>2</SUP>H<SUB>2</SUB>O solutions (pH 5.8±0.3, 5–72 °C by measuring <SUP>1</SUP>H NMR intensities, chemical shifts, coupling constants, and spin-lattice relaxation times. Below 50 °C, poly(C) exists as a slowly exchanging mixture of double-stranded and single-stranded forms of which only the single-stranded form is observable by <SUP>1</SUP>H NMR. In this temperature range, the single-stranded poly(C) shows a strong base-stacking with dominated <I>C</I><SUB>3′</SUB>-<I>endo anti</I> conformation, whereas above 50 °C it takes partly <I>C</I><SUB>2′</SUB>-<I>endo</I> conformation with destabilization of the helical structure. Poly(U) exists as single strand within the whole temperature range studied, and the bases are nearly unstacked with 58% of <I>C</I><SUB>2′</SUB>-<I>endo</I> conformer. Poly(U) is more flexible than poly(C), which was characterized with rotational correlation times in the order of 10<SUP>−9</SUP>–10<SUP>−10</SUP> s.
- 公益社団法人 日本化学会の論文
著者
-
Nosaka Yoshio
Department Of Chemistry Nagaoka University Of Technology
-
Akasaka Kazuyuki
Department Of Biotechnological Science School Of Biology-oriented Science And Technology Kinki Unive
-
Yamada-Nosaka Atsuko
Department of Chemistry, Faculty of Science, Kyoto University
-
Hatano Hiroyuki
Department of Chemistry, Faculty of Science, Kyoto University
-
Akasaka Kazuyuki
Department of Chemistry, Faculty of Science, Kyoto University
関連論文
- 2P126 Characterization of oligomeric human prion protein (23-231) by ^1H-NMR(31. Protein folding and misfolding (II),Poster Session,Abstract,Meeting Program of EABS & BSJ 2006)
- Photoelectrochemical Properties of the Sulfur-doped TiO_2 Film Electrodes : Characterization of the Doped States by Means of the Photocurrent Measurements
- Temperature Effect on the Selective Hydration of Sodium Ion in Nitrobenzene
- チタン酸化物を包含したゼオライトの評価とその光触媒的NO分解への応用〔英文〕
- Silane Coupling Agents for Photografting of Vinyl Monomer. Preparation and Properties of (N, N-Diethylamino)-dithiocarbamoylpropyl(trimethoxy)silane
- Photoinduced Changes of Surface and Adsorbed Water in TiO_2 Photocatalytic Systems as Studied by Solid State ^1H-NMR Spectroscopy
- Functional Tolerance of Streptomyces Subtilisin Inhibitor toward Conformational and Stability Changes Caused by Single-Point Mutations in the Hydrophobic Core
- Application of Chemiluminescent Probe to the Characterization of TiO_2 Photocatalysts in Aqueous Suspension
- Thermoluminescence of Gamma-Irradiated KCl
- Electron Spin Echo Investigation for Radicals Produced on ZnS Semiconductor Photocatalysts
- 2P123 The disulfide-bond dependence of the morphology of amyloid-like fibers from hen lysozyme(31. Protein folding and misfolding (II),Poster Session,Abstract,Meeting Program of EABS & BSJ 2006)
- 水溶液からの電解析出法によるCdS膜合成における光照射効果
- Dynamic structure of pyrimidine-polyribonucleotides in solution. 1HNMR studies.
- Phosphorescence Detection of the Singlet Molecular Oxygen Generated by Visible-light Irradiation on Gold-nanoparticle-deposited TiO_2 Photocatalysts
- Resonance Raman spectroscopic studies of electrogenerated cation radicals by column-electrolytic continuous-flow method.
- Direct determination of hyperfine coupling constants from complex and poorly resolved ESR spectra with numerical decoupling analysis.
- 31P magnetic relaxation in polynucleotides.
- The Behavior of Water Confined in Chemically Deteriorated PEFC Membranes as Studied by 1H NMR Spectroscopy
- Monitoring the Formation and Decay of Singlet Molecular Oxygen in TiO2 Photocatalytic Systems and the Reaction with Organic Molecules
- Chemical Modification of Subtilisin BPN' by 5-Diazo-1-H-Tetrazole
- Functional Tolerance of Streptomyces Subtilisin Inhibitor toward Conformational and Stability Changes Caused by Single-Point Mutations in the Hydrophobic Core.