Development and Application of the Serum Cholinesterase Activity by Ultra-Violet Detection Method Using Benzylthiocholine as Substrate

概要

論文の詳細を見る
We developed a method for the determination of serum pseudo-cholinesterase activity using benzylthiocholine as the substrate. The product thiocholine, which results from hydrolyzation of the substrate, was monitored using the ultraviolet light. The substrate and dithiodipyridine (2-PDS), the color-producing substance, were stable enough to make a ready-to-use reagent with them. Thiocholine breaks down the disulfide double bond of 2-PDS to produce 2- thiopyridone (2-TP). 2-TP has a maximum absorption at 343nm, and molar extinction coefficient of 679.0 mol<SUP>-1</SUP>·mm<SUP>-1</SUP>. Monitored by 340nm absorption, bilirubin and hemoglobin did not interfere with the assay. This new method is easily adaptable to automated analyzers and shows high specificity for pseudo-cholinesterase and good reproducibility in routine work.
Japan Society of Clinical Chemistryの論文