Studies on Human α1-Microglobulin XVl. Establishment of Radioimmunoassay of Double Antibody Method for Human α1-Micmglobulin and its Clinical Application

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we have established the double antibody radioimmunoassay for α1-microgloblin (α1-m).The range of reproducibilities for three various α1-m levels was 3.6 to 4.5% for within-assay variation and 4-0 to 8.0% for between-assay variation. The mean analytical recovery was 94.8% for serum α1-m and 97.0%for urinary α1-m, and reliable lirlearities were obtained in dilution tests.Correlation coefficients between the results by RIA and SRID for serum α1-m and those by RIA and EIA were 0.953 and 0.935, respectively.Using our method.the normal levels of serum α1-m were 18.8±4-4mg/l (mean±SD) in healthy males and 15.5±3.9mg/l in healthy females. Measurement of serum α1-m might be useful as a screening marker for diagnosis in early renal dysfunction and probably as a diagnostic tool for liver failure.