Investigation on Various Determination Methods of Serum Lipid Peroxide

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in the determination of lipid peroxide (LPo) by the method using 2-thiobarbituric acid (TBA) the addition of various metal ions to the reaction mixture containing authentic unsaturated fatty acids as sample led to a marked increase in LPO value. Metal ions showing a stimulation of LPO production were Fe2+, Fe3+, V3+ and Cu2+ and the orders of their stimulating activities were Fe2+>Fe3+>V3+>Cu2+.ln contrast, the metal ions such as Zn2+, Mg2+, Ca2+ and Mn2+ had no effect on the LPO production.ln the determination of serum LPO by the same method, however, Fe3+specifically caused an increase in LPO Value unlike the case on the authentic fatty acids. With respect to the correlation among the TAB method, the Fe3+-TBA method and the Hb-MB method, there was a significant correlation between the TBA method and the Fe3+- TBA method (p<0.001), while the Hb-MB method had no correlation with the remaining two methods. We further exammed the correlation between each determined value of serum components and serum LPO values estimated by the thre emethods. LPO values estimated by the TBA method were positively correlated with contents of total choiesterol, phospholipid and uric acid. LPO values estimated by the Fe3+-TBA method were positively correlated with contents of totalcho-esterol, phospholipid, triglyceride and albumin, and negatively correlated with LDH activity and ZTT level. LPO values estimated by the Hb-MB method showed a positive correlation with total protein content and uric acid values
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Investigation on Various Determination Methods of Serum Lipid Peroxide

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