Release of Several Enzymes from Rat Liver Lysosomes by Various Solubilizing Treatments

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Rat liver lysosomes were solubilized by treatments of detergents, hydrolyzing enzymes, sonication, freezing-thawing and hypotonicity with a sucrose solution. We investigated the effects of these treatments on the release of four enzymes, acid phosphatase (AcP), N-acetyl-β-D-glucosaminidase (NAG), β-glucuronidase (β-Glu) and acid deoxyribonuclease (AcDN), from the lysosomes.<BR>These four enzymes were solubilized to a similar extent by Triton X-100, a nonionic detergent. β-Glu and AcP were released more easily than NAG and AcDN by the treatment of cholic acid, an anionic detergent. In the case of trypsin digestion, the ratios of released AcDN and NAG were higher than those of released β-Glu and AcP. β-Glu was solubilized from the lysosomes in a ratio higher than the other enzymes by lipase digestion. In the treatment of sonication, there was little difference in the ratio of solubilization among these four enzymes. The treatment of freezing-thawing caused little release of NAG and AcDN but it had an apparent solubilizing effect on β-Glu and AcP in which their solubilization extents increased with an increase in freezing-thawing times. β-GIu was most easily solubilized by the treatment of hypotonicity with a sucrose solution.<BR>The present results indicate that β-Glu is one of the enzymes which are easily released from rat liver lysosomes by various solubilizing treatments.
Japan Society of Clinical Chemistryの論文