An Automated Technique for the Analysis of Serum Indolic Tryptophan Metabolites and Their Accumulation in Uremia

概要

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Separation and quantitation of 7 tryptophan metabolites in human serum within 50 minutes could be accomplished by high-performance liquid chromatography, with a fluorescence spectrophoto-meter as a detector. 0.6 molar acetate buffer (pH 3.8-6.5) was used for elution in a cation exchange column. Free and protein bound tryptophan metabolites in uremic and normal sera were measured by this method, and it was found that the concentration of free tryptophan in uremic serum was higher than in the normal while the concentration of total tryptophan was lower. ln all uremic sera, β-indole acetic acid and 5-hydroxyindole acetic acid were detected. and in some cases, 5-hydroxy tryptophol and N-acetyl tryptophan were also found. β-lndole acetic acid and 5-hydroxy indole acetic acid were found not only as free forms but also as protein bound forms. None of these indole compounds were detected in normal serum.
Japan Society of Clinical Chemistryの論文