Assay of Serum Cortisol by High Pressure Liquid Chromatography:(2) Responses of Serum Cortisol by Rapid ACTH Test

概要

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As previously reported1) human serum cortisol was assayed using high pressure liquid chromatography. After an intravenous single injection of 250μg of synthetic α<SUB>1-24</SUB> ACTH (Cortrosyn), serum cortisol level was followed until 180min passed, with endocrinologically healthy patients. The time-course curve of serum cortisol obtained by the pressent method, was almost consistent with the curve of a rapid ACTH test measured by the conventional fluorometric method2), except the values before ACTH and at 180min pased after ACTH were lower in our results. From these results the synthetic rate of cortisol was calculated, it showed that ACTH stimulates about 10 times the synthesis of cortisol. Also, the curves of abnormal responses against ACTH administration, such as non-reactive or delayed-type, were shown.