An Approach to Clinical Test of Mini-Column Chromatography with DEAE-Sephadex A-50 II. Determination of GOTm in Human Serum

概要

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A new mini-column method, which we devised previously for determination of IgG, was used for quantitative determination of the enzyme activities of GOTm in human serum. When 50μl of serum was applied to this mini-column with DEAE-Sephadex A-50, GOTm was not adosorbed and eluted rapidly. The caliblation curve of purified human GOTm was determined with Karmen's, Babson's and Reitman-Frankel method, and Karmen's method was the best one of these method. When several sera with addition of purified GOTm was applied to this column, GOTm was recovered about 100% in eluate. The correlation of this method and electrophoresis method was well at low activity of GOTm, but not well at high activity of GOTm by the reason why electrophoresis method can not determined GOTm accuratly. The correlation of this method and immunoadsorbance method was very well. This method thus appeares to be useful in daily clinical test.
Japan Society of Clinical Chemistryの論文